Figure 5
Sp8 induces Fgf8 downstream targets. (a-j) Induction of ectopic ETS TFs, Pea3, Erm and Er81 by the ectopic expression of Sp8. Coronal sections of E13.5 forebrains that were electroporated at E11.5 with Sp8 (a-e) or a control vector (f-j), mixed with EGFP, and processed for in situ hybridization using S35 riboprobes for Sp8 (b, g), Pea3 (c, h), Erm (d, i) and Er81 (e, j), respectively. EGFP (a, f) marks the electroporation domains (arrowheads). Pea3 (c), Er81 (d) and Erm (e) were induced by ectopic expression of Sp8. Scale bars: 0.5 mm.