Figure 9
Increased expression of isl or dHb9 in aCC/RP2 results in cell-specific perturbations in electrical properties. (A) Over-expression of isl in aCC/RP2 is sufficient to increase INa, reduce both IKfast and Ikslow while having no effect on ICa (-23.4 ± 1.6 versus -29.4 ± 2.2 pA/pF; -15.7 ± 1.6 versus -15.0 ± 3.23 pA/pF; 75.2 ± 3.0 versus 49.4 ± 5.7 pA/pF; 49.15 ± 5.75 versus 35.83 ± 3.13 pA/pF for INa, ICa, IKfast and Ikslow, respectively). (B) Ectopic expression of dHb9 in aCC/RP2, by contrast, results in a decrease in INa, no change in ICa and significant increases in both IKfast and IKslow (-23.4 ± 1.6 versus -14.0 ± 1.75 pA/pF; -15.7 ± 1.6 versus -16.4 ± 2.61 pA/pF; 54.1 ± 2.78 versus 57.51 ± 3.8 pA/pF; 34.7 ± 2.05 versus 42.15 ± 4.89 pA/pF for INa, ICa, IKfast and Ikslow, respectively). All values are mean ± SE, with islet (INa n = 7, ICa n = 7, IKfast n = 9, IKslow n = 7) and Hb9 (INa n = 9, ICa n = 6, IKfast n = 12, IKslow n = 7). *P ≤ 0.05, **p ≤ 0.01.