DamID demonstrates direct binding of Eve to slo and nAcRα-96Aa. The transcription unit of the gene of interest is shown in blue, and other transcription units in the region are shown in grey. Genes depicted above the line are encoded on the forward strand and those below are on the reverse strand. Exons are drawn in green. Vertical bars indicate the position of oligonucleotides on the genomic microarray. Bar heights are proportional to the average of normalized log-transformed ratio of intensities from two replicate DamID in vivo binding site mapping experiments. Genomic regions with a Dam-Eve/Dam ratio over 1.4 (red horizontal line) for at least four consecutive probes and with a deconvolved peak height greater than 1.8 were identified as Eve binding sites (red vertical bars).