Over-expression of eve reduces sensitivity to ACh. (A) Over-expression of eve in aCC/RP2 is sufficient to decrease the amplitude of AP-dependent synaptic currents in these neurons (89.2 ± 4.1 versus 76.0 ± 2.5, pA, *p ≤ 0.05). Synaptic currents were recorded, in voltage clamp, from a holding potential of -60 mV. The inset shows a typical recording of synaptic currents from a wild-type aCC neuron (note no differences were observed in current amplitude between aCC and RP2). (B) Over-expression of eve in aCC/RP2 also results in a significant decrease in the amplitude of AP-independent quantal synaptic currents (8.32 ± 0.26 versus 7.53 ± 0.22 pA, n = 10 cells (200 currents) and 7 (168 currents), respectively, *p ≤ 0.05). The inset shows a typical recording of minis in a control RP2 neuron (note no differences were observed in mini current amplitude between aCC and RP2). All values are mean ± SE (n ≥ 8). (C) Cumulative probability plot of amplitudes of AP-independent quantal synaptic currents shows that the majority are smaller in amplitude following over-expression of eve compared to control (RN2-O GAL4/+).