Fig. 1

Prdm12 interacts via its zinc fingers with the SET domain of G9a. A HEK293T cells were transfected with the indicated plasmids. Schematic diagrams of the Flag-PRDM12 and Myc-G9A WT and deletion mutants used are shown on the left. An empty FLAG plasmid was used as a control. Lysates were immunoprecipitated with anti-Flag antibodies. Immunoprecipitates and 5% of the input were then subjected to western blot analysis with anti-Flag or anti-Myc antibodies. B Full-length human Prdm12 or human glucocorticoid receptor (hGR) was synthesized in vitro and incubated with GST or GST fused to hG9a fragments bound to glutathione-agarose beads as indicated. Bound hPrdm12 and hGR proteins were detected by immunoblot with an anti-Flag antibody. A 2% input sample was loaded for comparison. The corresponding Commassie-stained gels are shown