Fig. 5

Neuronal identities in Aedes adult ventral ganglia. A Maximum intensity projections showing representative abdominal ganglia from segment A6 of 2-day post-eclosion brp-T2A-QF2w / +; QUAS-mcd8GFP / + adults stained with antibodies to Elav to label neurons and Hoechst to label all nuclei. B Quantification of neuronal number and total cell number across larval and adult development (3 days after larval hatching, 5 days after larval hatching, 2 days after adult eclosion). Bars depict mean values, points represent measurements from individual ganglia, and error bars represent standard deviation. N.s., not significant, one-way ANOVA analysis. C-E Distribution of GABAergic, serotonergic and dopaminergic neurons in adult abdominal VNC. Maximum intensity projection images depict the distribution of (C) GABAergic neurons (labeled by anti-GABA immunoreactivity), (D) serotonergic neurons labeled by anti-5HT immunoreactivity, and (E) dopaminergic neurons labeled by anti-TH immunoreactivity in segment A6 of 2-day post-eclosion adults. F-G Representative images depict TUNEL labeling of the larval (F) and adult abdominal ganglion (G). TUNEL labeling revealed very few apoptotic cells at either developmental stage. Fig. S17 depicts positive controls for labeling efficacy. H Schematic workflow schedule of EdU feeding and imaging. Animals were continuously maintained in EdU-containing water from Day 5 of larval development. I-J Representative images depicting distribution of anti-EdU immunoreactivity in abdominal ganglia from pupa (I) and 2-day post-eclosion adult (J). Although EdU incorporation was not readily detectable in abdominal ganglia, other tissues including tracheal cells (adjacent to ganglia) were readily labeled. Genotype: wild type in (I, J); all other panels: brp-T2A-QF2w / +; QUAS-mcd8GFP / +