Fig. 3

Identification of neuropeptide expressing cells. A Major neuropeptide-expressing cell clusters in the larval VNC. Heatmap depicts neuropeptide gene expression patterns in clusters that express neuropeptide genes in > 25% of cells at an average scaled expression of > 1.0. Cell cluster identity is indicated by numbers and horizontal bars above the heat map. B Feature plots depicting expression of neuropeptide genes ASTCC, burs, pburs, CCAP, Mip, and DH31 in cluster 19. Cells from this cluster co-express multiple neuropeptides. C Maximum intensity projections show Cluster 19 neurons identified in situ by immunostaining with antibodies to CCAP and pburs. All segments contain a bilaterally symmetric pair of neurons that co-express CCAP and pburs, and the posterior abdominal segment A6 but no other segment contains an additional pair of pburs-expressing cells that lack CCAP immunoreactivity. Yellow arrowheads mark CCAP+ pburs+ double-positive cells; white arrows mark CCAP- pburs+ cells. D FMRFamide expression is positively correlated with expression of the TF gene ct. Plot depicts expression levels of FMRFamide and ct in cluster 5. Dots represent individual cells, dot color represents pseudotime scores, and the linear regression line is shown in blue. E ct gene expression levels vary among different peptidergic clusters according to pseudo-time scores. Ct expression levels are plotted against pseudotime for the indicated peptidergic clusters. Dots represent individual cells and colors indicate cluster identity. Genotype for all panels: brp-T2A-QF2w / +; QUAS-mcd8GFP / +