Table 1 Key Resource Table
Reagent | Designation | Source | Identifiers | Additional information |
Species (D melanogaster) | 12E09-Gal4, 10xUAS-mCD8::GFP | Doe lab | n/a | Early INP driver |
Species (D melanogaster) | 16B06-Gal4, 10xUAS-mCD8::GFP | Doe lab | n/a | Late INP driver |
Species (D melanogaster) | UAS-ImpRNAi | BDSC | #34977 | Imp knockdown |
Species (D melanogaster) | UAS-Imp | MacDonald lab (UT Austin) | n/a | Imp overexpression |
Species (D melanogaster) | UAS-myr::GFP | BDSC | #32198 | Membrane bound GFP under UAS control |
Antibody | Chicken anti-GFP | Abcam (Eugene, OR) | n/a | 1:1000 |
Antibody | Rabbit anti-Imp | MacDonald lab (UT Austin) | n/a | 1:1000 |
Antibody | Rabbit anti-Syp | Genescript (Piscataway, NJ) | #4060 | 1:1000 |
Antibody | Rat anti-Dpn | Abcam (Eugene, OR) | n/a | 1:20 |
Antibody | Rat anti-Grh | Desplan lab (NYU) | n/a | 1:500 |
Antibody | Guinea pig anti-Scro | Genescript (Piscataway, NJ) | #7153 | 1:200 |
Antibody | Mouse anti-Elav | DSHB (Iowa City, IA) | 9F8A9-CM | 1:100 |
Antibody | Guinea pig anti-Pnt | Genescript (Piscataway, NJ) | #P0111 | 1:500 |
Antibody | Rabbit anti-DsRed | Rockland (Pottstown, PA) | #48710 | 1:500 |
Antibody, polyclonal | Secondary antibodies | Thermofisher (Eugene, OR) | n/a | 1:200 or 1:400 (Dpn and Scro only) |
Fly genotypes used in each figure | Figure | Synopsis | ||
; 37G12-LexA; 13xLexAop-myr::GFP; | Figure 1C | PF-R labeling | ||
; 60D05-LexA; 13xLexAop-myr::GFP; | Figure 1C | E-PG labeling | ||
;; 12E09-Gal4, 10xUAS-mCD8::GFP; | Figure 2A, B | INP lineage labeling | ||
;; 12E09-Gal4, 10xUAS-mCD8::GFP; | Figure 3B-M | INP lineage labeling | ||
;; 12E09-Gal4, 10xUAS-mCD8::GFP; | Figure 4B-M | INP lineage labeling | ||
;; 16B06-Gal4, 10xUAS-myr::GFP; | Figure 5A | oINP and nNeuron labeling | ||
;; 16B06-Gal4, 10xUAS-myr::GFP; x;; UAS-ImpRNAi | Figure 5B-J | oINP ImpRNAi and labeling | ||
;; 16B06-Gal4, 10xUAS-myr::GFP; x;; UAS-ImpOE | Figure 6A-I | oINP ImpOE and labeling | ||
; 60D05-LexA; x; 13xLexAop-myr::GFP; 16B06-Gal4 | Figure 7A-D | Control E-PG neurons | ||
; 60D05-Gal4; UAS-mCherryRNAi x; 13xLexAop-myr::GFP; 16B06-Gal4 | Not shown | Control E-PG neurons | ||
; UAS-mCherry; 60D05-LexA x; 13xLexAop-myr::GFP; 16B06-Gal4 | Not shown | Control E-PG neurons | ||
; 13xLexAop-myr::GFP; UAS-ImpRNAi x; 60D05-LexA; 16B06-Gal4 | Figure 7E-H | oINP ImpRNAi, E-PG labeling | ||
; UAS-Imp; 20xLexAop-DsRed x; 60D05-LexA; 16B06-Gal4 | Figure 7I-O | oINP ImpOE, E-PG labeling | ||
; 37G12-LexA; x; 13xLexAop-myr::GFP; 16B06-Gal4 | Figure 8A-D | Control PF-R neurons | ||
; 37G12-Gal4; UAS-mCherryRNAi x; 13xLexAop-myr::GFP; 16B06-Gal4 | Not shown | Control PF-R neurons | ||
; UAS-mCherry; 60D05-LexA x; 13xLexAop-myr::GFP; 16B06-Gal4 | Not shown | Control PF-R neurons | ||
; 13xLexAop-myr::GFP; UAS-ImpRNAi x; 37G12-LexA; 16B06-Gal4 | Figure 7E-H | oINP ImpRNAi, PF-R labeling | ||
; UAS-Imp; 20xLexAop-DsRed x; 37G12-LexA; 16B06-Gal4 | Figure 7I-M | oINP ImpOE, PF-R labeling | ||