Fig. 8
ImpRNAi and ImpOE disrupts PF-R neuropil targeting but not cell number. A-D Control confocal maximum intensity projection of PF-R neurons (A) and corresponding IMARIS rendering each targeted neuropil (A’-D). Scale bar 20 μm (A-C) or 10 μm (D). E-H ImpRNAi confocal maximum intensity projection of PF-R neurons (E) and corresponding IMARIS rendering each targeted neuropil (E’-H). Scale bar 20 μm (E-G) or 10 μm (H). I-O ImpOE confocal maximum intensity projection of PF-R neurons (I) and corresponding IMARIS rendering each targeted neuropil (I′-M); note that ImpOE results in PF-R neurons generating ectopic projections to the noduli (N). Scale bar 20 μm (I-K, M) 10 μm (L). N, O Quantification of cell numbers (N), and neuropil volume (O); each point represents an adult Drosophila brain, n = 3–5 brains in control, ImpRNAi, and ImpOE. Student t-tests were used to compare cell numbers to control. ANOVA analysis was used to compare neuropil volumes back to control. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001