Fig. 7
ImpRNAi and ImpOE alter E-PG neuropil targeting. A-D Control confocal maximum intensity projection of E-PG neurons (A) and corresponding IMARIS rendering each targeted neuropil (A’-D). Scale bar 20 μm (A-C) or 10 μm (D). E-H ImpRNAi confocal maximum intensity projection of E-PG neurons (E) and corresponding IMARIS rendering each targeted neuropil (E’-H). Scale bar 20 μm (E-G) or 10 μm (H). I-O ImpOE confocal maximum intensity projection of E-PG neurons (I) and corresponding IMARIS rendering each targeted neuropil (I′-O); note that ImpOE results in E-PG neurons generating ectopic projections to the fan-shaped body (FB), noduli (N), and mushroom body (MB). Scale bar 20 μm (J, K, M, N) or 10 μm (L, O). P, Q Quantification of cell numbers (P), and neuropil volume (Q); each point represents an adult Drosophila brain, n = 3–5 brains in control, ImpRNAi, and ImpOE. Student t-tests were used to compare cell numbers to control. ANOVA analysis was used to compare neuropil volumes back to control. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001