Fig. 5
16B06-gal4 > ImpRNAi knocks down Imp in oINPs. A 16B06-Gal4 > UAS-GFP UAS-ImpRNAi depletes Imp levels in oINPs, but not T2NBs at 48 h (B), 72 h (C), and 96 h (D). See Supplemental Fig. 1 for INP staging criteria. GFP marks oINPs and nNeurons. Scale bar 5 μm. B-G Confocal images of Imp levels in oINPs (B-D), quantified in E-G. Each point is a single oINPs, n = 3–5 brains per timepoint. H-M Confocal images of Imp levels in nNeurons (E-H), quantified in K-M). Each point is a single nNeuron, n = 3–5 brains per timepoint. Student t-tests were used to compare Imp levels at each timepoint. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001