Fig. 3

Imp forms a high-low gradient in 48 h INPs. A Quantification of Imp fluorescence in nINPs, yINPs, mINPs, oINPs and nNeurons at 48 h, 72 h, and 96 h. Note that Imp forms a high-low gradient in INPs at 48 h; later timepoints show INP age-specific expression. Each point represents a single INP, n = 3–5 brains per timepoint. ANOVA analysis was used to compare all cell types at each timepoint. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. B-M Confocal images of Imp levels in aging INPs at 48 h (B-E), 72 h (F-I), and 96 h (J-M). See Supplemental Fig. 1 for INP staging criteria. 12E09 > GFP marks the INP lineage beginning at yINPs. Scale bar 5 μm. N Summary