Fig. 2

Imp/Syp levels are the same in newborn INPs and T2NBs. A-F T2NBs (cyan circles; Pnt + GFP-) and nINPs (yellow circles, Pnt + GFP-, contacting T2NBs) at 48 h (A), 72 h (B), and 96 h (C). All timepoints have equivalent Imp (A-C) and Syp (D-F) values between T2NBs and nINPs. 12E09 > GFP marks the INP lineage starting at young INP stage. Scale bar 5 μm. G-H Quantification of Imp (G) and Syp (H) fluorescent levels in T2NBs and newborn INPs shows no significant differences at 48 h, 72 h, and 96 h. Each point is a single T2NB or nINP, with all 8 T2NBs included n = 3–5 brains per timepoint. Student t-tests were used to compare T2NBs and nINPs at each timepoint. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. I, K Quantification of average Imp (I) and Syp (J) levels in individual DM1–6 and DL1–2 T2NBs. Note that all T2NBs have a high-low gradient, whereas Syp shows a neuroblast-specific pattern of expression. n = 3–5 brains per timepoint. J T2NBs (cyan circles; Pnt + GFP-) at 48 h and 96 h in DM1 (J) and DM4 (J’). DM1 expresses Syp in a low-to-high expression gradient at 48 h to 96 h. DM4 Syp expression is the opposite high-to-low expression, similar to Imp. Scale bar 5 μm