Fig. 1

Transcriptional profiling of V0v spinal interneurons. Heatmap analysis of gene-expression profiling of 27 h V0v spinal cord interneurons. A three-class ANOVA analysis of differential expression was performed on different FAC-sorted populations of cells. Class 1: All trunk cells. Class 2: All post-mitotic spinal neurons. Class 3: V0v interneurons. Each column is a different biological replicate. Rows show relative expression levels for a single gene as normalized data transformed to a mean of 0, with standard deviation of + 1 (highly expressed, red) or -1 (weakly/not expressed, blue) sigma units. Adjusted P-values corrected for multiple testing are shown on the left-hand side. Expression profiles for positive control genes evx1 and evx2, whose spinal cord expression is exclusive to V0v interneurons, are shown. The high level of expression of these genes in our V0v samples, compared to the other samples, confirms that we have successfully isolated V0v interneurons. Additional positive control genes slc17a6a and slc17a6b, confirm that V0v interneurons are excitatory (glutamatergic), whereas negative control genes slc6a9, slc6a5, gad1b and gad2 show that V0v interneurons do not express either glycinergic or GABAergic inhibitory neurotransmitter pathway genes and that there is no contamination of our V0v samples with inhibitory neurons. The expression profiles for slc17a6a, slc17a6b, slc6a9, slc6a5, gad1b and gad2 are reproduced from [14] as per the Creative Commons Attribution (CC BY) license at Neural Development