Fig. 6

PCL NEPs migrate to the EGL and a subset of proliferating NEP-derived GCP-like cells migrate back into the cerebellar cortex in streams. a-b FIHC detection of CFP protein on mid-sagittal cerebellar sections (lobule II-III) of P8 Nes-CFP/+ (Nes-CFP, a) and Atoh1-Cre/+; Gli2^lox/lox; Nes-CFP/+ (Atoh1-Gli2 CKO; Nes-CFP, b) mice. The EGL is indicated by the yellow dotted lines and yellow arrows indicate the streams. c-d FIHC detection of the indicated proteins and dapi on high magnifications focusing on CFP+ streams of mid-sagittal cerebellar sections of P8 Atoh1-Cre/+; Gli2^lox/lox; Nes-CFP/+ (Atoh1-Gli2 CKO; Nes-CFP) mice. White arrows indicate co-localization of CFP with the indicated protein. e-g Detection of native CFP fluorescence on sagittal slice cultures of the vermis (lobule II-III, e and f and lobule I-II, g) of P8 Nes-CFP/+ (Nes-CFP, e) and Atoh1-Cre/+; Gli2^lox/lox; Nes-CFP/+ (Atoh1-Gli2 CKO; Nes-CFP, f and g) mice showing displacement of CFP+ cells during 6 h of imaging. Arrow color code is as indicated (red arrows indicates NEPs moving from PCL to EGL and green arrows indicates NEPs moving from EGL to IGL). The upper edge of the EGL is indicated by a yellow dotted line. Scale bars represent 100 μm (a-b and e-g) and 50 μm (c-d)