Fig. 5

Onecut1 dominant-negative induces L-Maf expression and a Rhodopsin reporter in the chicken retina, and is modulated by L-Maf activity. a-h Confocal imaged sections of retinas electroporated with CAG::Nuc β-gal, Rhodopsin::GFP, and either CAG::EnR (a-d) or CAG::OC1EnR (E-H). Retinas were immunostained with antibodies to GFP (green, chicken antibody), β-gal (red, mouse antibody), and L-Maf (magenta) and nuclei were stained with DAPI (blue). i-l Representative flow cytometry plots of dissociated cells from retinas electroporated with UbiC::TdT and Rhodopsin::GFP and the CAG construct(s) shown above each plot. The P6 gate demarks unambiguous double-positive cells. M) A bar graph of the percentage of Rho::GFP-positive cells in the electroporated population of retinas that were coelectroporated with the CAG construct(s) shown along the x-axis. Error bars represent standard error of the mean. Statistical analysis using Dunnett’s test for comparison of EnR group to 3 experimental groups. Significance value denoted by * = 0.1, ** = < 0.01 and N.S. signifies “No Significance”. N = 4 biological replicates for each condition. Scale bar in A represents 20 μm and applies to all image panels