Fig. 2

Candidate Brn3a target genes: Transcriptional and Translational regulators. In situ hybridization profiles for potential Brn3a target genes. Retinal sections from Rax:Cre; Brn3a^CKOAP/WT (left panel, WT) and Rax:Cre; Brn3a^CKOAP/KO (right panel, KO) mice, harvested at Postnatal days 0, 3, 7, 14 and 22 (P0, P3, P7, P14 and P22). The in situ hybridization probes were generated against the 3’-UTR of the corresponding target gene, using primers indicated in Table 1. A positive control (d, Brn3a) and a negative control (e, no probe) are shown. Bars on the right represent retina layers positions: black – NBL (neuroblast layer), red – GCL, cyan – IPL, blue – ONL, green – INL. a Rbfox1, b Foxp2, c Tshz2. Scale bar in (e), 50 μm