Fig. 5

N-cadherin is associated with actomyosin mechanical forces in differentiating cones in the pre-column zone and with Müller glia inter-column bands in the mature mosaic. (A-A”) Flat-mount of retinal margin, immunolabeled for ZO1 (magenta) and N-Cadherin (green). (B-B”’) Higher magnification of pre-column zone (boxes in A-A”). N-Cadherin labeling surrounds round cone profiles and is concentrated in adherens junction puncta (arrows) outside photoreceptor profiles. Immature rods are outlined by ZO-1 (B’, B”, yellow dots) but are negative for N-cadherin (B”). Large round profiles represent immature UV cones (B’, white dot). (C-C”) Flat-mount of retinal margin, immunolabeled for phospho-Myosin light chain II (magenta) and pan-Cadherin (green). (D-D”) Higher magnification of pre-column zone (yellow box); strong pMyoII and pan-Cadherin staining of round profiles of differentiating cones. (E-E”) Higher magnification of mature lattice mosaic (white box); pMyoII and pan-Cadherin co-localize to puncta between cone columns (arrows). Scale bar: 10 μm (A” and C”)