Fig. 8

Conversion of a dendrite to axonal polarity is faster after proximal axotomy than after distal. a Class IV ddaC neurons were subjected to proximal or distal axotomy and microtubule polarity in the dendrites was tracked 8 h and 24 h after injury. Neurons with at least one plus-end-out process were placed in the red category. Neurons with one or more mixed processes were placed in the grey category. Neurons with all minus-end-out processes were placed in the blue category. The graph shows percentage of proximally or distally axotomized neurons in each category at 8 h and 24 h after injury. b Class IV ddaC neurons were subjected to distal scarring axotomy and microtubule polarity in the dendrites was tracked 8 h and 24 h after injury. Neurons were placed in the same categories shown in a. A Fishers Exact test between the red categories in A and B was performed for the 24 h time-points (*p < 0.05). c Example of a Class IV ddaC neuron subjected to distal scarring axotomy and imaged 3 h after injury. The orange arrow shows the cut site. The same neuron was imaged 96 h after injury. The axon stump failed to re-grow. A dendrite converted to an axon and showed extensive growth as indicated by white arrow. d Out of 14 axotomized neurons, 9 neurons converted a dendrite to an axon, 2 neurons grew from the remaining axon stump and 3 neurons failed to grow from either location