Fig. 2.
From: Eph/Ephrin Signaling Controls Progenitor Identities In The Ventral Spinal Cord
EphrinB2 and ephrinB3 are expressed in complementary domains in progenitors of the ventral spinal cord. a-l. Transverse sections of Efnb2 +/GFP embryos at E8.5, E9.5, E10.5 and E11.5 (as indicated) were immunostained to detect Olig2 (e-h). Epifluorescence is shown on (a-d) and merged images are shown on (i-l). Scale bars: 50 μm. Dashed lines highlight the ventricular zone and brackets indicate domains of Efnb2:H2BGFP expression in progenitors. m-p. Transverse sections of Efnb2 +/GFP E11.5 embryos were immunostained to detect Olig2 (m) and Nkx2.2 (n). Epifluorescence is shown in (o) and a merged image is shown in (p). The dashed line marks the p3/pMN boundary. Scale bars: 50 μm. q-s. Transverse sections of wild type E10.5 embryos were processed for Olig2 immunostaining (q) and for Efnb3 in situ hybridization (r). A merged image is shown in (s). The dashed line marks the p3/pMN boundary. Scale bars: 25 μm. T. Schematic representation of Efnb2 and Efnb3 expression in relation to pMN and p3 progenitors domains at E11.5.