Fig. 1

Differential distribution patterns of E12.5- and E15.5-generated OSNs in the OE. a A coronal OE section from a postnatal day 0 (P0) mouse, immunostained with antibodies against OMACS (red) and OCAM (green). The OE section was counterstained with DAPI (blue). OMACS and OCAM are markers for the D- and V-zones, respectively. Schematic diagram of an OE section (shown on the right). b Distribution patterns of E12.5- and E15.5-generated OSNs in the OE. EdU and BrdU were injected at E12.5 and E15.5, respectively. OE sections from P0 mice were subjected to immunostaining with anti-BrdU antibodies and the Click reaction to detect EdU signals. c Enlarged images of the D- and V-zones of the OE. NCAM, an OSN marker, was detected by fluorescent in situ hybridization. d Percentages of OSNs generated at E12.5 and E15.5 were quantified in the OE. The OE sections were divided into ten subregions along with D-V axis. Percentages of labeled OSNs within the subregions were calculated as the number of labeled OSNs within each subregion divided by that in the entire OE region. Schematic diagram of the OE is shown on the right. n = 3 slices from 2 to 3 animals, error bars indicate SEM. Scale bars, 100 μm