Fig. 4
From: Netrin-1 directs dendritic growth and connectivity of vertebrate central neurons in vivo
Rapid remodeling of dendritic arbors upon acute manipulations in netrin signaling. a Schematic diagram of a stage 45 Xenopus tectal midbrain (horizontal view). Tectal neurons (red) make dendritic connections with contralateral RGC axons (green) within the tectal neuropil. b, c Sample RGC axons and tectal neurons, visualized by expression of GFP and tdTomato, respectively, in control (b) and netrin-treated (c) tadpoles. Note change in tectal neuron dendritic architecture evident at 4 and 24 h after netrin-1 treatment (inserts). d–g Confocal projections of representative tectal neurons co-expressing tdTomato (red) and PSD95-GFP (green) in tadpoles injected with control vehicle solution (d), Netrin (e), UNC5H2-Ig (f), or Netrin + UNC5H2-Ig (g). Note the emergence of an alternative primary dendrite (arrow) growing towards the midline in neurons exposed to netrin-1 or UNC5H2-Ig. Tadpoles treated with netrin + UNC5H2-Ig appeared identical to controls. Axons of tectal neurons are labeled by the asterisks. Scale bars: 20 μm