Figure 6

Vsx2 ^orJ RPCs do not produce RGC precursors in mutant chimeras at E12.5. (A-D) Expression of POU4F in retinas of wild type (A), orJ (B), control chimeras (C), and mutant chimeras (D) at E12.5. (C-inset, D-inset) Boxed areas in C and D. Dashed lines in B delineate the retina from adjacent lens tissue. Images are maximum Z-projections of confocal scans. (E) Bivariate graph showing the relationships between the percentages of EYFP− cells contributing to the RGC population (y-axis) and the percentages of EYFP− pixels contributing to the apical-basal extent of retinal tissue (x-axis) in the regions where POU4F+ cells were counted in mutant and control chimeras. Pixels serve as a proxy for relative contribution of EYFP− cells to the retina. Each data point was collected from a single retinal section (mutant chimeras: n = 9, 5 retinas; control chimeras: n = 11, 4 retinas) Scale bars: 100 μm (A, C); 40 μm (insets). Abbreviations: EYFP, enhanced yellow fluorescent protein.