Figure 6
From: Dynamic regulation of mRNA decay during neural development
PREs increase the rate of transcript decay in the nervous system. (A) Reporter construct design. Hey WT 3’ UTR contains the single endogenous PRE. Hey PRE Δ 3’ UTR has only the PRE sequence removed. (B) Decay curves based on reverse-transcription, quantitative-PCR measurements of reporter transcript decay (using GFP primers). Chase times are plotted on the x-axis (0-hour = end of 1-hour pulse). Mean chase/pulse values at the 1-hour chase and 3-hour chase time points are plotted based on multiple measurements. Half-lives in minutes are listed next to the decay curve for each transcript.