Figure 2

Adult expression of Pbx1a and En1 in mesencephalic dopaminergic neurons. In situ hybridization using digoxigenin-labeled riboprobes against (A) Pbx1a and (B) En1 on adult coronal sections and immunohistochemistry on adult mid-sagittal sections of En1+/tlZ mice using antibodies against (C) pan-Pbx and (D) ß-galactosidase to reveal the En1tauLacZ reporter gene. (A, B) Pbx1a and En1 expression co-localize in adult mesDA neurons. (C-E) Pbx1 protein (D, red) is detectable in the nucleus of all En1tauLacZ-expressing (C, green) mesDA neurons (E, arrowheads). Scale bars A, B = 250 μm and C-E = 200 μm . mesDA: mesencephalic dopaminergic.