Orthodenticle is necessary for survival of a cluster of clonally related dopaminergic neurons in the Drosophila larval and adult brain
© Blanco et al; licensee BioMed Central Ltd. 2011
Received: 1 June 2011
Accepted: 14 October 2011
Published: 14 October 2011
The dopaminergic (DA) neurons present in the central brain of the Drosophila larva are spatially arranged in stereotyped groups that define clusters of bilaterally symmetrical neurons. These clusters have been classified according to anatomical criteria (position of the cell bodies within the cortex and/or projection pattern of the axonal tracts). However, information pertaining to the developmental biology, such as lineage relationship of clustered DA neurons and differential cell subtype-specific molecular markers and mechanisms of differentiation and/or survival, is currently not available.
Using MARCM and twin-spot MARCM techniques together with anti-tyrosine hydroxylase immunoreactivity, we have analyzed the larval central brain DA neurons from a developmental point of view and determined their time of birth, their maturation into a DA neurotransmitter phenotype as well as their lineage relationships. In addition, we have found that the homeodomain containing transcription factor Orthodenticle (Otd) is present in a cluster of clonally related DA neurons in both the larval and adult brain. Taking advantage of the otd hypomorphic mutation ocelliless (oc) and the oc2-Gal4 reporter line, we have studied the involvement of orthodenticle (otd) in the survival and/or cell fate specification of these post-mitotic neurons.
Our findings provide evidence of the presence of seven neuroblast lineages responsible for the generation of the larval central brain DA neurons during embryogenesis. otd is expressed in a defined group of clonally related DA neurons from first instar larvae to adulthood, making it possible to establish an identity relationship between the larval DL2a and the adult PPL2 DA clusters. This poses otd as a lineage-specific and differential marker of a subset of clonally related DA neurons. Finally, we show that otd is required in those DA neurons for their survival.
The Drosophila adult brain is a highly organized and complex structure that contains thousands of neurons (in the order of 105)  exhibiting multiple cell-type identities, as characterized by various morphological, electrophysiological and molecular features. All these neurons arise from the mitotic activity of a small number of progenitor cells (neuroblasts (NBs)), which generate lineages of clonally related neurons via two proliferative phases of neurogenesis [2, 3]. The first phase of neurogenesis takes place during embryogenesis and starts with the specification and delamination of the NBs from the procephalic neurogenic region. Between embryonic stages 8 and late 11, around 100 NBs delaminate from this region on either side of the embryo in a reproducible spatiotemporal pattern . Each NB assumes a unique identity, as revealed by the expression of a specific set of marker genes such as the proneural genes, gap genes and segment polarity genes [5–7], and gives rise to an invariant cell lineage through multiple rounds of asymmetric cell divisions. In each cell division, the NB self-renews and generates a smaller daughter cell (named the ganglion mother cell), which divides only once to give rise to two post-mitotic neurons or glial cells (reviewed in [8–10]). The first neurogenic process terminates at the end of embryogenesis, when most NBs stop dividing and enter a dormant phase called quiescence . The cells so far generated (primary neurons) wire the larval nervous system and eventually may remodel during metamorphosis to contribute to the adult brain [12, 13]. The second phase of neurogenesis starts during the late first (L1) and second (L2) instar larval stage, when the inactive NBs resume mitotic activity and, through rounds of asymmetric cell divisions, generate the population of secondary neurons and glial cells that accounts for more than 90% of the adult brain [11, 14]. Hence, a complete NB lineage can be divided into two discrete cell populations, each containing the cells generated by the NB during distinct developmental phases (primary neurons during embryogenesis and secondary neurons during larval development) and each harboring multiple neuronal cell types. It has been proposed that neuron identity within a NB lineage depends on a combination of spatial and temporal cues provided, firstly, by the unique identity the NB acquires during its specification/delamination time [7, 15] and, secondly, as a result of a birth time/order-dependent mechanism , whereby cell-type specification of the nascent post-mitotic neurons depends on the identity of the progenitor temporal transcription factor expressed by the NB at each particular time during lineage progression .
The homeobox gene orthodenticle (otd), as a cephalic gap gene, is expressed in broad domains of the procephalic ectoderm during early neurogenesis, covering most of the protocerebral anlage and the anterior part of the deutocerebral anlage . Subsequently, its expression is also detected in NBs delaminating from these domains, where it plays instructive roles important for cell viability and spatial identity of the nascent NBs . It has also been proposed that otd might control brain NB formation by triggering proneural gene expression . Inactivation of otd at this early embryonic phase impairs NB formation and leads to a gap-like phenotype in the anterior head that includes the deletion of the protocerebral anlage and part of the deutocerebral anlage . Later in development, otd expression is also detected in post-mitotic neurons of the developing brain and ventral nerve cord, not only in the embryo, but also in the larval and pupal brain and even in mature neurons of the adult brain. In this regard, flies homozygous for an otd viable hypomorphic mutation called ocelliless (oc) show developmental defects that affect the protocerebral bridge, an important neuropile structure in the adult brain that is part of the central complex . However, whether this phenotype is due to the altered expression of otd in progenitor cells, post-mitotic cells or both is not known.
A comprehensive study of gene function during neuronal cell fate specification requires a previous and thorough cell lineage analysis and demands cell type-specific molecular markers to trace the cells under study. In this paper, we have focused our attention on the array of dopaminergic (DA) neurons that populate the Drosophila central brain during larval development. We have used cell lineage tracing genetic techniques together with immunoreactivity against the enzyme tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine biosynthesis [21, 22], to study their development, phenotypic maturation and lineage relationships. Interestingly, one cluster of clonally related DA neurons expresses otd from early L1 to adulthood, allowing us to examine the post-mitotic role of otd in controlling identity and/or survival of DA neurons in the Drosophila larval and adult brain.
Birth, clustering and phenotypic maturation of dopaminergic neurons in the larval central brain
We next determined the time of birth of the larval central brain DA neurons by using the MARCM (mosaic analysis with a repressible cell marker) technique in combination with the TH-Gal4 driver. Flippase recognition target (FRT)-mediated mitotic recombination was randomly induced, exposing progenitor cells to a one hour heat-shock treatment (37°C) at different developmental stages and L3 brains were assayed for the presence of green fluorescent protein (GFP)-labeled DA neurons. When mitotic recombination was induced during early L1, a time point commonly used to label neurons born during larval development, GFP-positive DA neurons were not detected in the resulting wild-type cell clones. This result agreed with the observation that TH expression was already detectable in early L1 brains (Figure 1H). On the contrary, a heat-shock treatment applied during early embryogenesis efficiently labeled DA neurons in the L3 central brain, with the highest labeling efficiencies achieved when cell clones were induced at early embryonic stages (between 3 and 7 and 7 and 11 hours after egg laying (AEL); Figure 1I). These results demonstrate that DA neurons present in the L3 central brain are primary neurons that arise during early embryogenesis.
Despite their early embryonic origin, the larval central brain DA neurons did not express the cell type-specific marker gene TH during embryogenesis, and even at late embryonic stages (stage 17) anti-TH immunoreactivity in the central nervous system was restricted to the ventral nerve cord (data not shown and ). However, during early L1 (24 to 28 h AEL) most of the central brain DA neurons already displayed TH expression (Figure 1H), with two exceptions: the DM1a DA neuron started to show anti-TH labeling during mid-late L1, whereas a DL2a DA neuron only showed anti-TH immunoreactivity at mid-late L3 (data not shown).
In summary, DA neurons present in the central brain of the Drosophila larva at L3 are generated during early embryogenesis and most of them acquire a mature neurotransmitter phenotype during early L1.
Dopaminergic neurons present in the central brain during larval development are generated by seven neuroblast lineages
In Drosophila, clonally related neurons typically remain clustered in the mature brain and project their neurites into specific neuropile compartments [26, 27]. To address whether the anatomical clustering of DA neurons in the L3 central brain is due to a clonal origin, we analyzed lineage relationships among DA neurons in each cell cluster. For this purpose, we utilized the twin-spot MARCM technique in combination with the tubulin-Gal4 driver. Wild-type cell clones were induced during early embryogenesis (3 to 7 h AEL) and assayed in early L1 brains (24 to 28 h AEL) for the presence of GFP- and red fluorescent protein (RFP)-labeled NB clones containing TH-positive neurons. Although at this developmental stage two DA neurons still did not express the cell type-specific marker gene TH and thus could not be considered in our analysis, two reasons justified our decision. Firstly, the reduced number of total cells present in the larval brain during L1 facilitated an easier lineage analysis. Secondly, we observed that the tubulin promoter underwent partial down-regulation in primary neurons during L3 (data not shown), impairing a reliable analysis of the lineage relationships among DA neurons.
As mentioned above, due to their delayed TH expression, two DA neurons were initially not included in the lineage analysis. In order to validate their allocation to the respective DA cell clusters, we induced MARCM-labeled wild-type NB clones during early embryogenesis (3 to 7 h AEL) and analyzed L3 brains. We tried to circumvent the down-regulation of the tubulin promoter by including an additional copy of the UAS-CD8::GFP transgene in the genotype of the analyzed larvae. Although this strategy did not always work reliably (for example, we did not observe single NB clones containing the group of six DL1 DA neurons), we were still able to assign the missing DM1 DA neuron to an independent NB lineage (named DM1a), as well as to confirm the allocation of the missing DL2 DA neuron to the DL2a NB lineage (Additional file 1).
In summary, we found that seven NB lineages generate the DA neurons present in each hemisphere of the Drosophila larval central brain and that their clustered appearance is, at least in part, a consequence of a clonal relationship. Moreover, the assignment of DA neurons to particular NB lineages provides access to study genetic mechanisms of DA neuron cell fate specification.
orthodenticle is expressed in DL2a dopaminergic neurons
To analyze the significance of otd expression in the specification and/or survival of post-mitotic DL2a DA neurons, we made use of the hypomorphic otd allele oc. oc hemizygous flies are viable, but they show a lack of ocelli and associated bristles in the head vertex. These flies also lack the protocerebral bridge, a neuropile structure present in the fly adult brain that is part of the central complex . These phenotypes arise as a consequence of chromosomal rearrangements that remove cis-acting regulatory sequences (oc enhancer) important for otd expression during ocelli and protocerebral bridge development [18, 28]. During early L1, otd expression in oc hemizygous larvae was downregulated and the number of TH-positive DL2a DA neurons was reduced to one or two neurons, as compared to the three DL2a DA neurons present in wild-type brains (Figure 3B). In L3 brains, the lack of anti-TH immunoreactivity affected three out of the four DL2a DA neurons (Figure 3D). To investigate whether this phenotype is due to the loss of DL2a DA neurons per se or to TH downregulation in these neurons, we made use of the oc enhancer and the oc2-Gal4 driver  as an alternative way of labeling DL2a DA neurons. Detection of oc enhancer activity in DL2a DA neurons in oc mutant L3 brains would imply that otd is involved in the activation and/or maintenance of TH expression in these neurons. By contrast, the absence of oc enhancer activity in DL2a DA neurons in oc mutant L3 brains would indicate otd is primarily necessary for survival of DL2a DA neurons. Reporter gene expression under the control of the oc2-Gal4 driver was detected in three out of the four DL2a DA neurons in wild-type L3 brains (Figure 3E'1-E'3). Interestingly, when oc2-gal4 transcriptional activity was analyzed in oc mutant L3 brains, reporter gene expression was not detected in DL2a DA neurons (Figure 3F,F1).
We also analyzed the relevance of otd expression during embryogenesis for the generation of DL2a DA neurons. We induced MARCM-labeled cell clones mutant for a null otd allele during early embryogenesis and assayed L3 brains for the presence of TH-positive cells within these otd mutant clones. Very few otd- NB clones were recovered in the central brain and none of them affected the DL2a DA neurons. However, L3 brains lacking some of the DL2a DA neurons were observed (white arrow in Figure 3G), though the corresponding NB clone was not detected. The simplest interpretation for this result is that Otd depletion during early neurogenesis induces NB cell death and, as a result, loss of DL2a DA neurons.
Taken together, the lack of anti-TH labeling observed in three DL2a DA neurons in oc mutant L3 brains is not due to TH downregulation, but likely reflects the absence of these neurons. Thus, we conclude that otd is necessary for survival of the larval DL2a DA neurons.
Adult PPL2 dopaminergic neurons derive from the larval DL2a cluster
Seven neuroblasts generate the larval central brain dopaminergic neurons
Otd acts as a survival factor in DL2a dopaminergic neurons
Most studies involving the homeodomain transcription factor Otd in central nervous system development in Drosophila have dealt with its role in the specification and proliferation of progenitor cells during early neurogenesis [5, 19], whereas a possible function in post-mitotic neurons has been largely overlooked. Our observation that otd is expressed in the DL2a DA neurons during larval development prompted us to investigate its role in the specification and/or survival of this DA cell cluster. According to anti-TH labeling, DL2a DA neurons mature mainly during early L1. Thus, null otd alleles, which are embryonic lethal, could not be used in our analysis. Therefore, we investigated the hypomorphic otd allele oc. We found that in oc mutant hemizygous larvae, otd expression in dorsolateral regions of the central brain was reduced and, as a consequence, only one of the four DL2a DA neurons showed anti-TH labeling during L3. The failure to detect three of the four DA neurons can be due to a defect in the regulation of TH expression or to the loss of DA neurons per se. Several lines of evidence support the latter hypothesis. Firstly, a general regulator of TH expression would be expected to be present in all or most of the central brain DA neurons; yet, otd expression during larval development is restricted to the DL2a DA cell cluster. Secondly, misexpression of otd in randomly induced cell clones in the central brain during larval development does not result in ectopic TH-expressing DA neurons (data not shown). Thirdly, labeling of DL2a DA neurons with the oc2-gal4 driver shows that reporter gene expression is also abolished in oc mutant hemizygous larvae during L3. The oc2 enhancer has been shown to be positively regulated by otd during ocelli development  and might not, therefore, be suitable to label DL2a DA neurons in an otd-independent way. However, a minimal version of this enhancer harboring the characterized Otd binding site (oc7) was active in the ocelli primordium , but did not show enhancer activity in DL2a DA neurons during larval development (data not shown). This indicates that the oc2 enhancer is differentially regulated in the ocelli primordium and in DA neurons during development and, hence, the oc2-gal4 driver may be used to label DL2a DA neurons in an otd-independent fashion.
Taken together, our observations support the hypothesis that otd expression is required for survival of DL2a DA neurons during larval development.
DL2a dopaminergic neurons survive into adulthood and participate in the PPL2 dopaminergic cell cluster
The wild-type Drosophila adult brain is populated by about 200 DA neurons distributed in several bilaterally symmetric clusters [23, 24, 29]. The PPL2 cluster contains seven cells that express otd and five of them also show oc2 enhancer activity in young adult brains (Figure 4A1-A4). Similarly to the larval brain, otd expression in PPL2 DA neurons seems to be necessary for their survival, since neither anti-TH immunoreactivity nor transcriptional activity of the oc2 enhancer is detected in oc mutant adult brains (Figure 4B and data not shown). Moreover, the effects of targeted depletion of Otd in PPL2 DA neurons (loss of cell viability and/or TH expression; Figure 4C) can be rescued by the simultaneous expression of the anti-apoptotic gene P35, pointing out a role in cell survival as the main function of otd in PPL2 DA neurons. Altogether, the simplest interpretation for these results would be that otd expression labels homologous DA neuron populations in both the larval (DL2a cell cluster) and adult (PPL2 cell cluster) brains and, hence, both clusters contain the same DA neurons. The discrepancy in cell number between both clusters of DA neurons can be interpreted by analyzing the NB lineage responsible for the generation of the DL2a DA neurons. At L3, this lineage contains seven otd expressing cells, four of them are primary neurons that have already undergone maturation and express TH. The other three cells might represent immature secondary neurons that differentiate during pupal stages to give rise to the additional three DA neurons present in the adult PPL2 cluster. The distinction between early-differentiating (four cells) and late-differentiating (three cells) PPL2 DA neurons finds support in the targeted depletion of Otd in DA neurons by RNAi. Expression of an otd-specific RNAi construct in DA neurons (using the TH-Gal4 driver) has no effect on the larval brain (data not shown), but impairs the viability of four PPL2 DA neurons in the adult brain. Since these four cells differentiate during larval development, the RNAi machinery would have more time to completely deplete Otd than in the case of the late differentiating DA neurons. Further support for this interpretation also comes from the analysis in the adult brain of wild-type twin-spot MARCM cell clones induced during early L1. According to this analysis, at least two PPL2 DA neurons in the adult brain are secondary neurons, whereas the third DA neuron might represent an undifferentiated primary neuron that only matures during pupal development.
Recently, the expression of Otx2, an otd ortholog, in DA neurons in the mouse adult brain has also been reported . It is selectively expressed in the central DA neurons of the ventral tegmental area, where it is cell autonomously required to antagonize identity features of the dorsal-lateral ventral tegmental area DA neurons . Thus, contrary to Drosophila, depletion of Otx2 in these DA neurons does not induce cell death, but it changes neuron subtype identity. Interestingly, otx2 expression in these DA neurons has been associated with their reduced vulnerability to Parkinsonian neurodegeneration .
Finally, in oc mutant adult flies most of the protocerebral bridge, a neuropile structure that is part of the central complex, is also missing . In several behavioral paradigms, these mutant flies walk slowly and show altered orientation behavior toward visual objects [36, 37]. It has been recently proposed that the protocerebral bridge is an essential part of a visual targeting network that transmits directional clues to the motor output . Thus, with regards to the data presented here, it would be interesting to analyze whether the lack of PPL2 DA neurons in oc mutant adult flies contributes to the behavioral phenotypes observed in these mutant flies.
Using MARCM and twin-spot MARCM techniques together with anti-TH immunoreactivity, we have classified the 21 DA neurons present in the Drosophila larval central brain into seven clusters of clonally related DA neurons. The homeobox gene otd is specifically expressed in DA neurons belonging to one of these clusters (DL2a cluster); thus, otd expression differentially labels a subset of DA neurons. Furthermore, by taking advantage of an otd hypomorphic mutation and the oc2-Gal4 reporter line, we have established a cell lineage relationship between the larval DL2a and the adult PPL2 DA cell clusters. We also studied the role of otd in the survival and/or cell fate specification of these post-mitotic neurons. Contrary to mice, where Otx2 expression in DA neurons of the adult brain is necessary for neuron subtype identity, otd is required in the Drosophila larval and adult brain for survival of DL2a and PPL2 DA neurons. These findings suggest that otd acts as a post-mitotic selector gene whose differential expression among DA neurons might help to establish functional differences.
Materials and methods
Fly strains, clonal analysis and RNAi experiments
Flies were reared on standard medium at 25°C. The following transgene and reporter lines were used: UAS-P35 (Bloomington Drosophila Stock Center, Bloomington, Indiana, USA), UAS-otd (J Blanco, unpublished), oc2-gal4 , TH-gal4 . Mutant alleles used in this study: ocγa1, otd YH13 .
Mitotic clones were generated and positively labeled (with membrane tethered CD8::GFP and CD2::RFP) according to the MARCM  and twin-spot MARCM  techniques. Unless indicated, recombination was induced 3 to 7 hours AEL by a one hour heat shock at 37°C and the larvae were dissected 21 hours later (early L1) or 110 hours later (L3). Genotypes of the analyzed larvae were as follows: otd YH13 MARCM clones, w otd YH13 FRT19A/w hs-FLP tubP-GAL80 LL1 FRT19A; tubP-GAL4 UAS-mCD8::GFP LL5 /+;
wild-type MARCM clones, y w hs-FLP/+; FRT82/FRT82 tubP-GAL80 LL10 ; tubP-GAL4 LL7 (or TH-GAL4) UAS-mCD8::GFP LL6 /UAS-mCD8::GFP LL6 ; wild-type twin-spot MARCM clones, y w hs-FLP/+; FRT40A UAS-mCD8::GFP UAS-CD2-Mir/FRT40A UAS-rCD2::RFP UAS-GFP-Mir; tubP-GAL4 LL7 (or TH-GAL4)/+.
Depletion of Otd by RNAi was carried out by targeted expression of an otd-specific RNAi construct (VDRC-105764) in DA neurons using the TH-gal4 driver. To increase knockdown efficiency, the experiment was done at 29°C in otd YH13 heterozygous flies.
Antibody staining on brains was performed as previously described . Primary antibodies were as follows: rabbit anti-Otd (1:250) , mouse anti-TH (1:100; Chemicon, Millipore AG, Temecula, California, USA), rabbit anti-TH (1:250) , rabbit anti-RFP (1:100; Abcam, Cambridge, UK). Secondary antibodies were Alexa488-, Alexa568- and Alexa647-conjugated antibodies generated in goat (1:200; Molecular Probes, Invitrogen, Paisley, Renfrewshire, UK). Fluorescent images were captured with an Olympus FV1000 confocal laser scanning microscope and analyzed in ImageJ . Unless otherwise indicated, pictures correspond to single optical sections (1 μm thick). Figures were assembled using Adobe Illustrator and Photoshop.
after egg laying
flippase recognition target
green fluorescent protein
instar larval stage
mosaic analysis with a repressible cell marker
- oc :
- otd :
protocerebral posterior lateral
red fluorescent protein
We acknowledge B Bello, C Desplan, T Lee, B Lu, U Walldorf and the Bloomington Stock Center for kindly providing fly strains and reagents. This work was supported by the Joint Singapore Bioimaging Consortium (SBIC)-Singapore Stem Cell Consortium (SSCC).
- Ito K, Awasaki T: Clonal unit architecture of the adult fly brain. Adv Exp Med Biol. 2008, 628: 137-158. 10.1007/978-0-387-78261-4_9.View ArticlePubMedGoogle Scholar
- Urbach R, Technau GM: Neuroblast formation and patterning during early brain development in Drosophila. Bioessays. 2004, 26: 739-751. 10.1002/bies.20062.View ArticlePubMedGoogle Scholar
- Hartenstein V, Spindler S, Pereanu W, Fung S: The development of the Drosophila larval brain. Adv Exp Med Biol. 2008, 628: 1-31. 10.1007/978-0-387-78261-4_1.View ArticlePubMedGoogle Scholar
- Urbach R, Schnabel R, Technau GM: The pattern of neuroblast formation, mitotic domains and proneural gene expression during early brain development in Drosophila. Development. 2003, 130: 3589-3606. 10.1242/dev.00528.View ArticlePubMedGoogle Scholar
- Urbach R, Technau GM: Molecular markers for identified neuroblasts in the developing brain of Drosophila. Development. 2003, 130: 3621-3637. 10.1242/dev.00533.View ArticlePubMedGoogle Scholar
- Urbach R, Technau GM: Segment polarity and D/V patterning gene expression reveals segmental organization of the Drosophila brain. Development. 2003, 130: 3607-3620. 10.1242/dev.00532.View ArticlePubMedGoogle Scholar
- Technau GM, Berger C, Urbach R: Generation of cell diversity and segmental pattern in the embryonic central nervous system of Drosophila. Dev Dyn. 2006, 235: 861-869. 10.1002/dvdy.20566.View ArticlePubMedGoogle Scholar
- Egger B, Chell JM, Brand AH: Insights into neural stem cell biology from flies. Philos Trans R Soc Lond B Biol Sci. 2008, 363: 39-56. 10.1098/rstb.2006.2011.PubMed CentralView ArticlePubMedGoogle Scholar
- Knoblich JA: Mechanisms of asymmetric stem cell division. Cell. 2008, 132: 583-597. 10.1016/j.cell.2008.02.007.View ArticlePubMedGoogle Scholar
- Doe CQ: Neural stem cells: balancing self-renewal with differentiation. Development. 2008, 135: 1575-1587. 10.1242/dev.014977.View ArticlePubMedGoogle Scholar
- Truman JW, Bate M: Spatial and temporal patterns of neurogenesis in the central nervous system of Drosophila melanogaster. Dev Biol. 1988, 125: 145-157. 10.1016/0012-1606(88)90067-X.View ArticlePubMedGoogle Scholar
- Truman JW: Metamorphosis of the central nervous system of Drosophila. J Neurobiol. 1990, 21: 1072-1084. 10.1002/neu.480210711.View ArticlePubMedGoogle Scholar
- Truman JW: Steroid receptors and nervous system metamorphosis in insects. Dev Neurosci. 1996, 18: 87-101. 10.1159/000111398.View ArticlePubMedGoogle Scholar
- Prokop A, Technau GM: The origin of postembryonic neuroblasts in the ventral nerve cord of Drosophila melanogaster. Development. 1991, 111: 79-88.PubMedGoogle Scholar
- Skeath JB, Thor S: Genetic control of Drosophila nerve cord development. Curr Opin Neurobiol. 2003, 13: 8-15. 10.1016/S0959-4388(03)00007-2.View ArticlePubMedGoogle Scholar
- Kao CF, Lee T: Birth time/order-dependent neuron type specification. Curr Opin Neurobiol. 2010, 20: 14-21. 10.1016/j.conb.2009.10.017.PubMed CentralView ArticlePubMedGoogle Scholar
- Jacob J, Maurange C, Gould AP: Temporal control of neuronal diversity: common regulatory principles in insects and vertebrates?. Development. 2008, 135: 3481-3489. 10.1242/dev.016931.View ArticlePubMedGoogle Scholar
- Finkelstein R, Smouse D, Capaci TM, Spradling AC, Perrimon N: The orthodenticle gene encodes a novel homeo domain protein involved in the development of the Drosophila nervous system and ocellar visual structures. Genes Dev. 1990, 4: 1516-1527. 10.1101/gad.4.9.1516.View ArticlePubMedGoogle Scholar
- Younossi-Hartenstein A, Green P, Liaw GJ, Rudolph K, Lengyel J, Hartenstein V: Control of early neurogenesis of the Drosophila brain by the head gap genes tll, otd, ems, and btd. Dev Biol. 1997, 182: 270-83. 10.1006/dbio.1996.8475.View ArticlePubMedGoogle Scholar
- Hirth F, Therianos S, Loop T, Gehring WJ, Reichert H, Furukubo-Tokunaga K: Developmental defects in brain segmentation caused by mutations of the homeobox genes orthodenticle and empty spiracles in Drosophila. Neuron. 1995, 15: 769-778. 10.1016/0896-6273(95)90169-8.View ArticlePubMedGoogle Scholar
- Budnick V, White K: Catecholamine-containing neurons in Drosophila melanogaster: distribution and development. J Comp Neurol. 1988, 268: 400-413. 10.1002/cne.902680309.View ArticleGoogle Scholar
- Nässel DR, Elekes K: Aminergic neurons in the brain of blowflies and Drosophila dopamine- and tyrosine hydroxylase-immunoreactive neurons and their relationship with putative histaminergic neurons. Cell Tissue Res. 1992, 267: 147-167. 10.1007/BF00318701.View ArticlePubMedGoogle Scholar
- Monastirioti M: Biogenic amine systems in the fruit fly Drosophila melanogaster. Microsc Res Tech. 1999, 45: 106-121. 10.1002/(SICI)1097-0029(19990415)45:2<106::AID-JEMT5>3.0.CO;2-3.View ArticlePubMedGoogle Scholar
- Friggi-Grelin F, Coulom H, Meller M, Gomez D, Hirsh J, Birman S: Targeted gene expression in Drosophila dopaminergic cells using regulatory sequences from tyrosine hydroxylase. J Neurobiol. 2003, 54: 618-627. 10.1002/neu.10185.View ArticlePubMedGoogle Scholar
- Selcho M, Pauls D, Han KA, Stocker RF, Thum AS: The role of dopamine in Drosophila larval classical olfactory conditioning. PLoS ONE. 2009, 4: e5897-10.1371/journal.pone.0005897.PubMed CentralView ArticlePubMedGoogle Scholar
- Truman JW, Schuppe H, Sheperd D, Williams DW: Developmental architecture of adult-specific lineages in the ventral CNS of Drosophila. Development. 2004, 131: 5167-5184. 10.1242/dev.01371.View ArticlePubMedGoogle Scholar
- Pereanu W, Hartenstein V: Neural lineages of the Drosophila brain: a three-dimensional digital atlas of the pattern of lineage location and projection at the late larval stage. J Neurosci. 2006, 26: 5534-5553. 10.1523/JNEUROSCI.4708-05.2006.View ArticlePubMedGoogle Scholar
- Blanco J, Seimiya M, Pauli T, Reichert H, Gehring WJ: Wingless and Hedgehog signaling pathways regulate orthodenticle and eyes absent during ocelli development in Drosophila. Dev Biol. 2009, 329: 104-115. 10.1016/j.ydbio.2009.02.027.View ArticlePubMedGoogle Scholar
- Mao Y, Davis RL: Eight different types of dopaminergic neurons innervate the Drosophila mushroom body neuropil: anatomical and physiological heterogeneity. Front Neural Circuits. 2009, 3: 5-PubMed CentralView ArticlePubMedGoogle Scholar
- Lee T, Luo L: Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron. 1999, 22: 451-461. 10.1016/S0896-6273(00)80701-1.View ArticlePubMedGoogle Scholar
- Wu JS, Luo L: A protocol for mosaic analysis with a repressible cell marker (MARCM) in Drosophila. Nat Protoc. 2006, 1: 2583-2589.View ArticlePubMedGoogle Scholar
- Bello BC, Izergina N, Caussinus E, Reichert H: Amplification of neural stem cell proliferation by intermediate progenitor cells in Drosophila brain development. Neural Dev. 2008, 3: 5-10.1186/1749-8104-3-5.PubMed CentralView ArticlePubMedGoogle Scholar
- Yu HH, Chen CH, Shi L, Huang Y, Lee T: Twin-spot MARCM to reveal the developmental origin and identity of neurons. Nat Neurosci. 2009, 12: 947-953. 10.1038/nn.2345.PubMed CentralView ArticlePubMedGoogle Scholar
- Di Salvio M, Di Giovannantonio LG, Omodei D, Acampora D, Simeone A: Otx2 expression is restricted to dopaminergic neurons of the ventral tegmental area in the adult brain. Int J Dev Biol. 2010, 54: 939-945. 10.1387/ijdb.092974ms.View ArticlePubMedGoogle Scholar
- Di Salvio M, Di Giovannantonio LG, Acampora D, Prosperi R, Omodei D, Prakash N, Wurst W, Simeone A: Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP. Nat Neurosci. 2010, 13: 1481-1488. 10.1038/nn.2661.View ArticlePubMedGoogle Scholar
- Strauss R: The central complex and the genetic dissection of locomotor behaviour. Curr Opin Neurobiol. 2002, 12: 633-638. 10.1016/S0959-4388(02)00385-9.View ArticlePubMedGoogle Scholar
- Triphan T, Poeck B, Neuser K, Strauss R: Visual targeting of motor actions in climbing Drosophila. Curr Biol. 2010, 20: 663-668. 10.1016/j.cub.2010.02.055.View ArticlePubMedGoogle Scholar
- FlyBase. [http://flybase.bio.indiana.edu]
- Bello B, Holbro N, Reichert H: Polycomb group genes are required for neural stem cell survival in postembryonic neurogenesis of Drosophila. Development. 2007, 134: 1091-1099. 10.1242/dev.02793.View ArticlePubMedGoogle Scholar
- Hirth F, Kammermeier L, Frei E, Walldorf U, Noll M, Reichert H: An urbilaterian origin of the tripartite brain: developmental genetic insights from Drosophila. Development. 2003, 130: 2365-2373. 10.1242/dev.00438.View ArticlePubMedGoogle Scholar
- Yang Y, Gehrke S, Imai Y, Huang Z, Ouyang Y, Wang JW, Yang L, Beal MF, Vogel H, Lu B: Mitochondrial pathology and muscle and dopaminergic neuron degeneration caused by inactivation of Drosophila Pink1 is rescued by Parkin. Proc Natl Acad Sci USA. 2006, 103: 10793-10798. 10.1073/pnas.0602493103.PubMed CentralView ArticlePubMedGoogle Scholar
- Image Processing and Analysis in Java. [http://rsbweb.nih.gov/ij/]
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.