The long noncoding RNA Six3OS acts in trans to regulate retinal development by modulating Six3 activity
© Rapicavoli et al; licensee BioMed Central Ltd. 2011
Received: 28 May 2011
Accepted: 21 September 2011
Published: 21 September 2011
Thousands of different long non-coding RNAs are expressed during embryonic development, but the function of these molecules remains largely unexplored.
Here we characterize the expression and function of Six3OS, a long non-coding RNA that is transcribed from the distal promoter region of the gene encoding the homeodomain transcription factor Six3. Overexpression and knockdown analysis of Six3OS reveals that it plays an essential role in regulating retinal cell specification. We further observe that Six3OS regulates Six3 activity in developing retina, but does not do so by modulating Six3 expression. Finally, we show that Six3OS binds directly to Ezh2 and Eya family members, indicating that Six3OS can act as a molecular scaffold to recruit histone modification enzymes to Six3 target genes.
Our findings demonstrate a novel mechanism by which promoter-associated long non-coding RNAs can modulate the activity of their associated protein coding genes, and highlight the importance of this diverse class of molecules in the control of neural development.
It has recently become clear that long non-coding RNAs (lncRNAs) comprise a large fraction of the mammalian transcriptome . Much effort has been focused on functional analysis of lncRNAs that are processed into short fragments, such as microRNAs, that regulate expression of protein coding genes via homologous base pairing. However, several thousand mammalian lncRNAs have been identified that span multiple kilobases in length, and in some cases show extensive conservation at the nucleotide level [2–4].
To date, only a small number of lncRNAs have been functionally characterized, although this list is growing rapidly. Some lncRNAs act via antisense base pairing to block gene expression [5–7], but many show no clear sequence overlap with the mRNAs of protein coding genes. Several of these lncRNAs are known instead to regulate mRNA transcription, acting in cis to regulate heterochromatin formation at nearby genomic loci. The Xist/Tsix transcripts mediate X-inactivation in placental mammals , and Kcnq1ot is important for silencing of the Kcnq locus resulting from parental imprinting . Other lncRNAs regulate transcription of genes that are located great distances away from their own genomic loci. One notable example of such a trans-acting lncRNA is HOTAIR, which is transcribed from within specific Hox gene clusters, but which regulates the expression of Hox genes located on different chromosomes [10, 11]. HOTAIR, Kcnq1ot and Xist all mediate their effects by interacting with the Polycomb-repressive complex 2 (PRC2) component Ezh2 (enhancer of zeste homolog 2 (Drosophila)) and modulating histone methylation [9, 11, 12]. Finally, a small number of lncRNAs also directly interact with transcription factors, and potentially function as transcriptional coregulators [13–15]. Although the emerging picture suggests that lncRNAs may play an important and widespread role in regulating mammalian gene expression, a central and still unresolved question is how lncRNAs act in trans to regulate expression of specific target genes without the use of homologous base paring.
A complex assortment of lncRNAs is expressed in the developing and mature mammalian central nervous system, with the cellular expression patterns of nearly 1, 000 different lncRNAs having been previously described [16–18]. Many show highly specific expression in specific brain regions and neuronal subtypes and it has been speculated that these lncRNAs may play a critical role in generating and maintaining the great cellular complexity found in the central nervous system [19, 20]. Although a limited number of intergenic lncRNAs have been found to regulate neural development, their mode of action remains obscure [21, 22]. Mechanistic insight into the function of one brain-expressed lncRNAs has come from analysis of Evf-2, a lncRNA co-transcribed with the homeodomain factor Dlx6. Evf-2 modulates transcription of Dlx6 by recruiting DLX2 and MECP2 to the ultraconserved ei enhancer element that is also transcribed as part of Evf-2 itself. The transcribed domain containing the ei sequence is essential for Evf-2 to activate expression of Dlx6, which has raised the possibility that Evf-2 might regulate Dlx6 transcription at least in part through the formation of a RNA-DNA hybrid; this hybrid may in turn facilitate binding of the ei sequence by DLX2 and MECP2 [14, 15].
Recent studies have also begun to address the function of long non-coding opposite-strand transcripts (lncOSTs), which are divergently co-transcribed with a broad range of neuronally expressed genes. Over one-third of brain-expressed homeodomain genes possess an associated lncOST, which typically spans the promoter, but not the transcribed region, of the protein coding gene in question [23, 24]. Since short promoter-associated ncRNAs can regulate expression of nearby protein coding genes [25–27], this has raised the possibility that these lncOSTs might also act in cis to selectively regulate the expression of their associated protein coding gene. However, although lncOSTs comprise a substantial fraction of all brain-expressed lncRNA species, their function has yet to be directly investigated.
In this study, we characterize the molecular function and mechanism of the lncOST Six3OS. Six3OS is co-expressed with the homeodomain factor Six3, a homologue of the Drosophila sine oculis gene [28, 29]. Like sine oculis, Six3 plays a critical role in mammalian eye development, regulating both early eye formation and cell specification in the postnatal retina [30, 31]. Both Six3 and Six3OS are strongly and selectively expressed in the developing mouse retina and hypothalamus [23, 32, 33]. We use both in vivo overexpression and short hairpin RNA (shRNA)-mediated knockdown analysis to analyze whether gain or loss of function of Six3OS results in altered differentiation of specific retinal cell subtypes. We also examine whether Six3OS acts cooperatively with Six3 to regulate retinal differentiation, but find that Six3OS does not regulate Six3 expression levels. Finally, we provide evidence that Six3OS can directly bind both to known transcriptional coregulators of Six3 and to histone modification enzymes, thereby functioning as an RNA-based transcriptional scaffold. These results demonstrate the mechanism by which this diverse class of molecules regulates cell specification during development.
Genomic organization of Six3 and Six3OS
Cellular expression pattern of Six3OS
Overexpression and knockdown of Six3 and Six3OS
Since Six3OS was coexpressed with Six3 in retinal progenitors, we hypothesized that Six3OS might regulate the expression and/or activity of Six3 in developing retina. To determine if this was indeed the case, we employed in vivo electroporation of neonatal (P0.5) mice to determine whether overexpression and knockdown of Six3OS phenocopied the effects of overexpression and knockdown of Six3. For overexpression of Six3OS, we used full-length cDNAs corresponding to BC065087 (Figure 1) cloned into the pCAG plasmid . Upon transfection into HeLa cells, robust Six3OS expression was confirmed by FISH (Additional file 1). To visualize electroporated cells, all constructs were co-electroporated with plasmids expressing green fluorescent protein (GFP) from the same CAG promoter.
We next tested the effects of overexpression of pCAG-Six3 using in vivo electroporation. Upon electroporation, expression of Six3 was confirmed by section immunohistochemistry. Cell fate specification in the postnatal retina is highly sensitive to Six3 dosage, and both gain and loss of function of Six3 have been reported to result in similar defects in bipolar cell and photoreceptor development . We observed that electroporation of 1 μg of pCAG-Six3 led to a reduction in the number of rod bipolar cells, and detected an increase in the fraction of GFP-positive amacrine cells by P21 (P < 0.05; Figure 3A, D). The length of the outer segments of GFP-positive rod photoreceptors was also decreased (P < 0.05; Figure 3D, G). Finally, we observed that the cell bodies of the Six3 electroporated photoreceptor cells were primarily located in the outer third of the outer nuclear layer (P < 0.05; Figure 3D, white dashed line, and 3F), in contrast to photoreceptors electroporated with control vector, which were distributed throughout the outer nuclear layer.
We then analyzed the effects of loss of function of Six3OS and Six3. To this end, we first tested shRNAs for their ability to reduce expression of endogenous Six3 and Six3OS expression by in vivo electroporation of P0.5 retina. Reduction in expression of the target gene was determined by analyzing expression of either Six3 protein or Six3OS RNA dissociated GFP-positive cells. For both Six3 and Six3OS, individual shRNA constructs were identified that resulted in a substantial reduction in the average fluorescence intensity in either Six3 protein or Six3OS RNA in GFP-positive cells (Additional file 2).
Simultaneous overexpression and knockdown of Six3 and Six3OS demonstrates non-additive effects on retinal differentiation
Having observed that loss of function of Six3 and Six3OS resulted in similar phenotypes in developing retina, we next investigated whether these two genes acted cooperatively or independently to regulate retinal differentiation. We first used in vivo electroporation to overexpress 1 μg of both the Six3 and Six3OS constructs simultaneously in P0.5 retina. We observed that co-expression of Six3 and Six3OS resulted in a normal cell fate phenotype (Figure 3A). The length of GFP-positive rod photoreceptor outer segments was indistinguishable from controls (Figure 3B, E, G). However, the cell bodies of the electroporated photoreceptor cells were primarily located in the outer third of the outer nuclear layer (P < 0.05; Figure 3B, E, white dashed line, and 3F). We thus concluded that overexpression of Six3OS was largely able to reverse the cellular phenotypes observed following overexpression of Six3.
As a follow up to these experiments, we simultaneously reduced expression of Six3 and Six3OS by co-electroporating 1 μg of the Six3 shRNA construct and 1 μg of Six3OS shRNA construct. We observed that simultaneous knockdown of both Six3 and Six3OS resulted in a normal number of bipolar cells and Muller glia. However, we observed a significant decrease in the fraction of GFP-positive amacrine cells, which was not observed following electroporation of either the Six3 or Six3OS shRNA alone (P < 0.05; Figure 4A, E). These data show a non-additive effect of simultaneous loss of function of both Six3 and Six3OS, and show that these genes functionally interact to regulate retinal differentiation.
In contrast, when 1 μg of CAG-Six3OS was co-electroporated with 1 μg of Six3 shRNA, we observed an increase in glutamine synthetase-positive cells with Muller glia-like morphology (P < 0.05; Figure 5B) and a decrease in PKCα-positive and CHX10-positive bipolar cells (P < 0.05; Figure 5B, C, E) when compared to controls. Additionally, a decrease in cells expressing the amacrine cell-specific marker syntaxin was observed (P < 0.05; Figure 5B). These retinas, however, do not show any change in the number of GFP-positive cells with amacrine cell-like morphology. We therefore conclude that, as is the case when Six3OS alone was overexpressed, Six3OS overexpression in conjunction with knockdown of Six3 reduces syntaxin expression without otherwise affecting amacrine cell differentiation (Figure 5B, E). The phenotype seen here is the sum of the phenotypes seen following Six3OS overexpression and Six3 knockdown, and stands in contrast to the non-additive phenotype seen when Six3OS and Six3 are both simultaneously knocked down (Additional file 4). We thus conclude that the phenotype observed following Six3OS overexpression is not affected by Six3 loss of function, and that Six3OS may also regulate syntaxin expression through a Six3-independent mechanism.
Six3OS does not directly regulate Six3 protein levels
Although these data suggest that Six3OS regulates Six3 function in developing retina, they do not directly address the molecular mechanism by which Six3OS is able to do so. A number of studies have suggested that both promoter and enhancer-associated lncRNAs can act in cis to regulate expression levels of nearby protein-coding genes [14, 25–27, 35, 36]. To investigate whether Six3OS might be regulating Six3 function by a similar mechanism, we overexpressed and knocked down expression of Six3OS at P0.5 in retina, and quantified the level of Six3 protein in GFP-positive cells at P5.5. We did not observe any change in Six3 protein levels following either overexpression or knockdown of Six3OS (Additional file 5A, B). Furthermore, we tested whether Six3OS could regulate Six3-dependent regulation of a reporter construct derived from the sequence of the mouse Six3 promoter, which was previously shown to be negatively regulated by Six3 . To determine if Six3OS modulates Six3-mediated auto-repression, CAG-Six3OS was cotransfected with the CMV-Six3 and the Six3-pro luciferase vector. As previously reported, overexpression of Six3 represses this reporter construct when tested in NIH3T3 cells. However, Six3OS overexpression had no effect on Six3-mediated auto-repression (Additional file 6). Although 14 additional human and mouse cell lines were tested, no Six3OS-dependent effects on Six3-mediated autorepression could be detected (data not shown). We conclude that transcription of Six3 mRNA is not regulated by Six3OS. Instead Six3OS RNA likely modulates the ability of Six3 protein to activate or repress expression of its target genes in developing retinal cells.
Ezh2 and Eya1/3/4 directly bind Six3OS
To identify proteins that directly bind Six3OS, Cy5-labeled Six3OS RNA was used to probe human protein microarrays . Mouse Six3OS and three human splice variants of Six3OS were transcribed in vitro and labeled with Cy5, and antisense cRNAs for these same transcripts were hybridized in parallel as negative controls. A total of five proteins that specifically and selectively interact with both the mouse and human forms of Six3OS were thus identified. We found that both human and mouse Six3OS selectively interact with Eya1, encoded by a homologue of the eyes absent gene of Drosophila. In addition, Six3OS directly binds to the chromatin remodeling enzyme subunits Smarce1 and Ezh2, as well as Eno1 and Ppp5c (Additional file 7). Members of the Eya protein family have been previously shown to interact with Six family proteins , but Eya1 is not expressed in the mouse neuroretina . Therefore, we also performed RNA immunoprecipitation (RIP) experiments on Eya3 and Eya4, which are both robustly expressed in embryonic retina.
Six3OS and Six3 modulate retinal cell specification and differentiation
Our findings demonstrate that both gain and loss of function of Six3OS and Six3 affect retinal differentiation (Additional file 4). Knockdown of Six3OS resulted in a decrease in the fraction of bipolar cells and an increase in Muller glia in electroporated cells. An identical phenotype was observed following knockdown of Six3 (Figure 4). Knockdown of either Six3OS or Six3 phenocopies the effect of retroviral overexpression of a dominant-negative form of Six3 in neonatal retina, where a substantial decrease in bipolar cells was also seen .
In contrast, overexpression of either Six3 or Six3OS alone produced very different phenotypes. Six3OS overexpression resulted in a reduction in syntaxin staining in electroporated amacrine cells, but did not alter the morphology or laminar position of electroporated cells. On the other hand, Six3 overexpression, like Six3 loss of function, resulted in a dramatic reduction in the number of bipolar cells (Figure 3A), and also resulted in decreased rod photoreceptor outer segment length at P21 (Figure 3D, G). These effects were previously reported following retroviral-mediated overexpression of Six3. Interestingly, we also observed phenotypes that were not reported in this previous study, such as an increase in amacrine cells. A number of technical differences between these two experiments may account for these differences, most notably the fact that electroporation-mediated overexpression typically results in much higher levels of construct expression in developing retina than does retroviral transduction .
Six3OS regulates Six3 activity in developing retina, but does not regulate Six3 expression levels
If Six3 and Six3OS act independently to control retinal cell specification, we would expect to observe purely additive phenotypes when their activities are both altered. Strikingly, when both Six3OS and Six3 were overexpressed simultaneously, we no longer observed the cellular phenotypes that resulted from overexpression of Six3 alone. Simultaneous knockdown of both Six3OS and Six3 also showed non-additive effects. The loss of bipolar cells and increase in Muller glia, which was seen following knockdown of each gene individually, were not observed when expression of both Six3 and Six3OS was reduced. Instead, a decrease in amacrine cells was observed, which was a phenotype not observed when each individual gene was knocked down. These non-additive phenotypes demonstrate an epistatic relationship between Six3 and Six3OS, and imply that they functionally interact to regulate retinal differentiation.
Having demonstrated that Six3 and Six3OS interact to regulate retinal differentiation, we next investigated whether defects in Six3 could be rescued by overexpression of Six3OS, and vice versa. When Six3 is overexpressed but Six3OS is knocked down, none of the phenotypes normally seen following either Six3 overexpression alone or Six3OS knockdown alone were observed (Figure 5A, C-D). These data indicate that loss of function of Six3OS suppresses the effects of both gain and loss of function of Six3. However, simultaneous overexpression of Six3OS and knockdown of Six3 resulted in a purely additive phenotype that was the sum of the effects of Six3 knockdown and Six3OS overexpression. These findings suggest that Six3OS acts to regulate Six3 activity, since overexpression of Six3 can rescue the effects of Six3OS knockdown, but not vice versa. As Six3OS overexpression leads to a reduction in syntaxin expression even following Six3 knockdown, this implies that Six3OS may also regulate retinal differentiation through a Six3-independent mechanism, although this remains to be characterized further.
Since both promoter- and enhancer-associated ncRNAs have been previously thought to act in cis to selectively regulate expression of nearby protein coding genes [36, 39], our finding that Six3OS did not regulate Six3 expression came as something of a surprise. Our finding that Six3OS acts in trans to regulate retinal differentiation was supported by several lines of evidence. First, by the finding that the activity of Six3 expressed from the CAG promoter can be modulated by overexpression or knockdown of Six3OS (Figure 5). Second, we have directly demonstrated that neither overexpression nor knockdown of Six3OS has any detectable effect on Six3 expression in retina, nor does Six3OS have an effect on Six3-dependent autorepression when measured by luciferase analysis in transfected tissue culture cells. These data imply that Six3OS and perhaps other lncOSTs do not regulate transcription of their associated protein coding genes, but instead act in trans to regulate the activity directly via protein-RNA interactions. This mechanism may not hold true for all promoter-associated lncRNAs, however, and each will have to be characterized independently to determine its mechanism of action.
Six3OS selectively interacts with Eya family members and Ezh2
While these data demonstrate a functional relationship between Six3OS and Six3, they still leave the precise mechanism by which Six3OS regulates Six3 activity unresolved. Our finding that both human and mouse isoforms of Six3OS interact with multiple different members of the eyes absent protein family demonstrates how this might occur in vivo. The eyes absent (eya) gene and its mammalian homologues encode protein tyrosine phosphatases that function as transcriptional coregulators. Eya binds directly to sine oculis, and acts in conjunction with sine oculis in controlling eye field specification in Drosophila[28, 29]. We also observe that Six3OS directly binds the PRC2 subunit Ezh2, and also possibly the BAF57 subunit Smarce1. Additional confirmation that Six3OS interacts with Ezh2 in vivo comes from a recent study of polycomb-associated RNAs, which found that Six3OS was co-precipitated with PRC2 in embryonic stem cells . We propose that Six3OS can modulate the expression of Six3 target genes without necessarily regulating expression of Six3 itself by acting as a transcriptional scaffold. When Six3 directs the Six3-Eya complex to bind to specific genomic target sequences, Six3OS may thus act as a transcriptional scaffold, recruiting histone modifying enzyme complexes to regulate expression of Six3 target genes. Although trans-acting lncRNAs have previously been found to regulate gene expression through recruitment of the Ezh2-containing PRC2 histone methyltransferase complex, their action is restricted to a small subset of target genes, and the mechanism by which this occurs is unknown . Our findings point towards a plausible mechanism by which this could occur. However, in the absence of RNA-IP data that clearly demonstrate interaction of Six3OS with both Eya proteins and Six3 in developing retina, other potential mechanisms of action of Six3OS remain plausible.
Several unresolved questions remain, the most obvious being which Eya subtype is the most relevant target of Six3OS in vivo. Eya1 is known to bind Six3 both in vitro and in transfected tissue culture cells , although the co-expression of Six3 and Eya1 is limited to the ciliary margin and lens . Eya2, Eya3 and Eya4, however, are all co-expressed with Six3 and Six3OS in both the developing retina and in other forebrain regions . Eya4 and Six3 have been demonstrated to interact in a study of holoprosencephaly, indicating that Eya4 may be the functional bridge between Six3 and Six3OS in the developing ventral forebrain . Our findings indicate that Six3OS may also regulate retinal differentiation by Six3-independent mechanisms. Since Eya family members can interact with other classes of transcription factors, including Hox and Tlx family members , this suggests that Six3OS might also modulate the expression of genes regulated by these transcription factors.
Many homeodomain transcription factors that are essential for central nervous system development possess an associated lncOST, including Pax6, Rax, Vax2 and Six6 [23, 45]. Each of these proteins exhibits complex and dynamic expression during development, and their expression patterns often diverge considerably from those of their associated lncOST. Although their target sequences are present in all cells, the genes that are directly regulated by each protein can vary considerably, both at different developmental stages and in different cell types. Models that explain how this context-dependent regulation of transcription factor activity occurs have typically emphasized combinatorial regulation by other proteins, or cell- and stage-specific epigenetic modifications. Our finding that lncOSTs can modulate the activity of their associated transcription factors lends an additional layer of complexity to these models, and suggests that this diverse class of molecules may play a critical function in generation of cell subtype diversity within the developing central nervous system.
Materials and methods
Pregnant CD-1 mice were purchased from Charles River Breeding Laboratories, Wilmington, MA, USA. Animal experiments were approved by the Johns Hopkins University Institutional Animal Care and Use Committee.
In vivo electroporation
In vivo electroporation of mouse retina was performed as described . Retinas were electroporated at P0.5. Dissociated cells and section immunohistochemistry data shown here were performed at P21. Error bars represent ± standard error of the mean for at least three independent electroporated retinas. A two-tailed Students t-test was performed to determine the P-value. Control and experimental constructs were co-electroporated with 0.2 μg of pCAG-GFP to readily visualize electroporated cells by GFP . For overexpression experiments, 1 μg of pCAG-GFP was injected with either 1 μg of pCAG-Six3 or 1 μg of CAG-Six3OS. For simultaneous overexpression of Six3 and Six3OS, retinas were electroporated with 1 μg of pCAG-Six3 and 1 μg of CAG-Six3OS. For knockdown of Six3 and Six3OS, 1 μg of U6-control was injected with either 1 μg of U6-Six3 or 1 μg of U6-Six3OS. For simultaneous knockdown, 1 μg of U6-Six3 and 1 μg of U6-Six3OS were injected. For overexpression of Six3 in combination with knockdown of Six3OS, retinas were electroporated with 1 μg of pCAG-Six3 and 1 μg of U6-Six3OS were injected. Finally, for overexpression of Six3OS in combination with knockdown of Six3, 1 μg of pCAG-Six3OS and 1 μg of U6-Six3 were injected and electroporated.
In situ hybridization
For cryosections, untimed E14.5 and E16.5 embryos and P0.5 neonates were dissected, fixed overnight in 4% paraformaldehyde in PBS at 4°C and cryoprotected overnight in 30% sucrose/PBS at 4°C before being embedded in OCT compound (Sakura, Torrance, CA, USA) on dry ice. Cryosections (20 μm) were cut on a cryostat. Section ISH methodology was as previously described  with the exception that probe BC065087 was used and a Tyramide Signal Amplification system (TSA Plus, Perkin Elmer, NEL 744, Waltham, MA, USA) combined with an antidigoxigenin-HRP antibody (1:1, 000; Roche, Indianapolis, IN, USA). for fluorescent ISH. Fluorescent ISH sections were counterstained with DAPI. Fluorescent ISH samples were photographed on a Zeiss Meta 510 confocal microscope. Chromogenic images were visualized on a Zeiss Axioskop2 microscope.
For cryosections, electroporated eyes were harvested 21 days after electroporation, fixed for 1 hour in 4% paraformaldehyde in PBS at 4°C and cryoprotected overnight in 30% sucrose/PBS at 4°C before being embedded in OCT compound (Sakura) on dry ice. Cryosections (20 μm) were cut on a cryostat. Retinal cryosections were immunostained as described except that sections were post fixed in 4% paraformaldehyde for 5 minutes prior to blocking . Samples were photographed on a Zeiss Meta 510 confocal microscope.
For cell marker analysis, dissociation and immunostaining were performed as described  except that retinas were harvested at P21. Samples were visualized and quantified on a Zeiss Axioskop2 microscope. To quantify the effects of Six3 knockdown, retinas were electroporated with Six3 shRNA, harvested at P4.5, dissociated, and immunostained as described above with anti-Six3 and detection with Alexa568 goat anti-guinea pig IgG and rabbit anti-GFP and detection with Alexa488 goat anti-rabbit. For Six3 quantification, Six3OS overexpression and SixOS shRNA electroporated retinas were harvested at P5.5, dissociated and immunostained as described above with anti-Six3 and detection with Alexa568 goat anti-guinea pig IgG and rabbit anti-GFP and detection with Alexa488 goat anti-rabbit. Samples were visualized on a Zeiss Axioskop2 microscope and signal intensity was quantified with Velocity 4.0 (Perkin Elmer) software by calculating the average signal intensity per cell and normalized to cell size.
In situ hybridization and immunocytochemistry
For Six3OS knockdown quantification, retinas were harvested at P4.5 and dissociated as described above. FISH was performed as described using TSA Plus Cyanine3 kit (1:125; Perkin Elmer, NEL 744) followed by staining as described (with rabbit anti-GFP and detection with Alexa488 goat anti rabbit). Samples were visualized and quantified as above. For all dissociations, nuclear DNA was visualized with DAPI counterstaining. Cell counts were analyzed using the two-tailed Student's t-test. A minimum of three retinas were counted for each construct examined using dissociated immunocytochemistry, with 100 to 300 GFP-positive cells per retina counted for each marker tested.
HeLa cell FISH followed by immunocytochemistry was performed as previously described  except that 20 cells were counted.
Luciferase reporter assays
NIH 3T3 cells were grown in DMEM supplemented with 10% FCS. Cells were transfected with Fugene6 (Roche) per the manufacturer's instructions. Cells were transfected with 500 ng of luciferase reporter construct and 50 ng of the expression plasmids for Six3 and Six3OS. Cells were harvested 2 days post-transfection. Luciferase was measured per manufacturer's instructions with the Dual-Luciferase Reporter System (Promega, E1910, Madison, WI, USA). pTK-Renilla (50 ng) was co-transfected to control for transfection efficiency.
Synthesis of RNA probes for protein microarray
BC065087 (5 μg) was linearized with DraI, and 5 μg of BM663835, BX115070, and BM451513 were linearized with NotI. Linearized DNA was then phenol-chloroform extracted, ethanol precipitated and resuspended in 10 μl water. Probes were in vitro transcribed with T7 polymerase per manufacturer's instructions using the Riboprobe Combination system kit (Promega, P1405), spiking the reaction with 1 μl of 5 mM Cy5 labeled CTP (GE Healthcare, 25-8010-87, Piscataway, NJ, USA). The RNA probe was ethanol precipitated with LiCl and resuspended in 1 mM EDTA.
Protein microarray analysis
Transcription factor/RNA binding protein chips were generated as previously described . Protein chips were preblocked with Superblock Buffer (Thermo Scientific, 37516, Waltham, MA, USA) supplemented with 10 μg ml-1 sperm DNA, 2 mM MgCl2, 2 mg ml-1 BSA for 1 hour at room temperature. RNA was hybridized at 250 nM concentration in binding buffer (PBS supplemented with 2 mM MgCl2, 2 mg ml-1 BSA) at room temperature for 1 hour. The slides were washed four times in TBST, dried and scanned by a GenePix 400B scanner. Data were analyzed with GenePix Pro 6.10 as previously described .
RNA immunoprecipitation experiments
HEK 293T cells were grown in DMEM supplemented with 10% FCS. Cells were transfected with Fugene6 (Roche) per the manufacturer's instructions. Ten million cells were transfected 24 hours post-plating with 5 μg of pCAGIG-V5, pCAGIG-Six3-V5, pCAGIG Eya1-V5, pCAGIG Eya3-V5, pCAGIG Eya4-V5 or pCAGIG Ezh2-V5, and pCAG-Six3OS. Cells were harvested 2 days post-transfection, lysed and precipitated essentially as previously described , except that 5 μg anti-V5 antibody was used (1:5, 000; Invitrogen, R96025, Carlsbad, CA, USA) with 50 μl protein A-agarose (Invitrogen, 15918-014) in each immunoprecipitation reaction. For each sample, 10% of total volume was set aside after lysis for RNA extraction and 5% set aside for immunoblot analysis. After precipitation, 80% of the beads were resuspended in Trizol and 20% were resuspended in Laemmle buffer. RNA was Trizol extracted and resuspended in 50 μl of nuclease free water. RNA was then DNAse treated with Turbo DNA-free (Ambion, AM1907, Carlsbad, CA, USA) per manufacturer's instructions. RNA was quantified by quantitative RT-PCR using Brilliant II SYBR Green QRT_PCR Master Mix (Agilent, 600825, Santa Clara, CA, USA) on a Roche LightCycler480. No-RT controls were simultaneously performed to demonstrate that signal was not from DNA contamination.
Expression of V5-tagged protein was confirmed by western analysis using anti-V5 antibody (1:10, 000; Invitrogen, R96025) dilution in 5% milk in PBST, and detected with horse radish peroxidase goat anti-mouse IgG (1:10, 000; Santa Cruz, sc-2031, Santa Cruz, CA, USA) and ECL Western Blotting Detection System (GE Healthcare, RPN2132 Piscataway, NJ, USA) per the manufacturers' instructions.
Full details of all plasmids and antibodies used in this study are included in Additional File 9.
bovine serum albumin
Dulbecco's modified Eagle's medium
fetal calf serum
fluorescent in situ hybridization
green fluorescent protein
in situ hybridization
long non-coding opposite-strand transcript
long non-coding RNA
protein kinase C
Polycomb-repressive complex 2
short hairpin RNA
Tris- buffered saline with Tween-20.
The authors thank Shiming Chen, Tomomi Shimogori, Jeremy Nathans, Wendy Yap and members of the Blackshaw lab for comments on the paper. This work was supported by a grant from the Whitehall Foundation to SB and a VNTP training grant to EP. SB is a WM Keck Foundation Distinguished Young Scholar in Medical Research.
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