Figure 5
From: Sequential generation of olfactory bulb glutamatergic neurons by Neurog2-expressing precursor cells
Neurog2 progenitors generate juxtaglomerular neurons with distinct morphologies at E17.5 and P0. (A,B) Maximal intensity projection of Neurog2-derived juxtaglomeular neurons generated at E17.5 (A) and P0 (B). Note the distinct morphology of the neurons generated at the two time points. While most neurons generated at earlier embryonic time points present intraglomerular dendritic arborization, those generated at birth show periglomerular dendritic arborization. (C) Maximal intensity projection of Tbr1-positive (arrowhead) and Tbr1-negative (arrow) neurons. (D-G) Other illustrative examples of maximal intensity projection showing the dendritic arborization of Tbr1-negative (D,E) and Tbr1-positive (F-G) neurons. (H,I) Tbr1 immunoreactivity identifies juxtaglomerular neurons with distinct morphologies as illustrated by the smaller dendritic arbor (H), while no differences in their branching ratio could be observed (I). Scale bars: 50 μm in (A-C); 10 μm in (C-F). Error bars indicate S.E.M.; *P < 0.05, determined by unpaired t-test.