FGF dependent regulation of Zfhx1b gene expression promotes the formation of definitive neural stem cells in the mouse anterior neurectoderm
© Dang and Tropepe; licensee BioMed Central Ltd. 2010
Received: 11 January 2010
Accepted: 6 May 2010
Published: 6 May 2010
Mouse definitive neural stem cells (NSCs) are derived from a population of LIF-responsive primitive neural stem cells (pNSCs) within the neurectoderm, yet details on the early signaling and transcriptional mechanisms that control this lineage transition are lacking. Here we tested whether FGF and Wnt signaling pathways can regulate Zfhx1b expression to control early neural stem cell development.
By microinjecting FGF8b into the pro-amniotic cavity ex vivo at 7.0 days post-coitum (dpc) and culturing whole embryos, we demonstrate that neurectoderm-specific gene expression (for example, Sox2, Nestin, Zfhx1b) is increased, whereas Wnt3a represses neurectoderm gene expression. To determine whether FGF signaling also mediates the lineage transition from a pNSC to a NSC, 7.0-dpc embryos were microinjected with either FGF8b or inhibitors of the FGF receptor-MAP kinase signaling pathway ex vivo, cultured as whole embryos to approximately 8.5 dpc and assayed for clonal NSC colony formation. We show that pre-activation of FGF signaling in the anterior neurectoderm causes an increase in the number of colony forming NSCs derived later from the anterior neural plate, whereas inhibition of FGF signaling significantly reduces the number of NSC colonies. Interestingly, inhibition of FGF signaling causes the persistence of LIF-responsive pNSCs within the anterior neural plate and over-expression of Zfhx1b in these cells is sufficient to rescue the transition from a LIF-responsive pNSC to an FGF-responsive NSC.
Our data suggest that definitive NSC fate specification in the mouse neurectoderm is facilitated by FGF activation of Zfhx1b.
Mouse neural induction depends on synergistic interactions among three primitive tissues: anterior ectoderm, anterior visceral endoderm, and anterior mesendoderm derivatives of the early gastrula organizer . Transforming growth factor (TGF)β/bone morphogenetic protein (BMP), Wnt and fibroblast growth factor (FGF) are three major signaling pathways known to function during these complex tissue interactions and insight into the relative contribution of each of these pathways for early development has been gleaned from targeted deletion studies. For example, the absence of Wnt3a results in ectopic neural tissue formation at the expense of paraxial mesoderm derivatives , and the loss of the TGFβ-related signaling components Smad4, Bmpr1a or Nodal in the primitive ectoderm leads to precocious neural differentiation and a loss of pluripotency. Together, these data suggest that the attenuation of Wnt, BMP and Nodal signaling pathways are necessary for neural cell fate specification of the mouse anterior ectoderm, a mechanism that is broadly conserved among vertebrates . The requirement for FGF signaling is less apparent. For instance, the Fgf4 and Fgfr2 null mutations are embryonic lethal during blastocyst implantation [7, 8]. Furthermore, mouse embryos harboring Fgfr1-/- or Fgf8-/- mutations, which die at approximately 8.5 to 9.5 days post-coitum (dpc), indicated that FGF signaling was not required for the earliest steps in neural specification of the ectoderm, although it was necessary for normal gastrulation and neurulation [9–11]. In contrast, studies in chick  and Xenopus provide evidence for an early role of FGF signaling in specifying a neural identity. Thus, it is possible that the early pleiotropic effects of FGF signaling may act to partially obscure a specific localized role during mouse neural cell fate specification.
Embryonic stem (ES) cell models of neural induction have been instrumental in complementing the mouse in vivo studies . Indeed, Wnt and/or BMP inhibition within ES cell cultures results in a clear enhancement of neural specification and neuronal differentiation. For example, inhibition of Wnt signaling using DKK1  or Sfrp2  showed enhanced neural differentiation. Similarly, the BMP antagonist Noggin can enhance neural cell fate specification of ES cell cultures in serum-free and feeder-free conditions , whereas in the presence of a potent neural inducing feeder cell line, exogenous BMP4 can suppress neural induction . These in vitro findings are consistent with the in vivo evidence linking the inhibition of BMP and Wnt signaling with mouse neural cell fate specification .
In contrast, there are conflicting views over whether FGF acts as a positive inducing signal or whether it plays a more permissive role during neural cell fate specification of mouse ES cells. For example, blocking FGF signaling pharmacologically or genetically under low cell density, serum-free and feeder-free conditions does not prevent ES cells from acquiring a neural cell identity after 24 hours . However, many of the neural cells are specified to a 'primitive' neural state, whereby they express primarily neuroectodermal genes (for example, Sox1, Nestin), rather than mesodermal or endodermal genes, but also maintain low levels of expression of the pluripotency gene Oct4. The term 'primitive' reflects the fact that although these cells are specified to a neural identity, they are not yet committed (that is, definitive) . On the other hand, others have shown that ES cells harboring an Fgf4-/- or Erk2-/- mutation are significantly deficient in their capacity to be induced to a definitive neural identity (Sox1+, Nestin+, Oct4-) . In these experiments, however, neural induction was typically assayed over the course of several days, making it difficult to completely rule-out a permissive role for FGF signaling on proliferation of cells specified to a neural identity independently of FGF . An alternative model, which would reconcile these somewhat disparate conclusions, posits that FGF signaling is required for the transition from a primitive neural identity to a definitive neural identity within the ectoderm during neural plate formation in vivo, or during ES cell differentiation in vitro, irrespective of its influence on proliferation.
Previous studies have reported that the anterior ectoderm (approximately 5.5 to 7.5 dpc) contains primitive neural stem cells (pNSCs), which when isolated in clonal in vitro assays display self-renewal properties, express neural genes and have the potential to generate differentiated neuronal and glial progeny . pNSC-derived colonies differ from definitive neural stem cell (NSC) colonies in two main respects: they require exogenous leukemia inhibitory factor (LIF) for colony formation in primary cultures, instead of FGF; and they express relatively low levels of the pluripotency gene Oct4, which enables a broader differentiation potential under the influence of non-neural tissue environments [17, 23]. Definitive NSCs, first evident at 8.5 dpc in the anterior neural plate, require FGF to stimulate clonal colony formation from primary dissections and are committed to a neural identity . Thus, the differential growth factor requirement for colony formation serves as a useful feature to distinguish between these two stem cell populations during the course of NSC lineage ontogeny.
In the present study we tested the hypothesis that FGF signaling is required for the transition from a pNSC identity to a NSC identity by manipulating FGF signaling directly within the mouse ectoderm ex vivo and then assaying for the presence or absence of pNSC and NSC clonal colony-formation from stem cells isolated from the neural plate. We report that the activation of FGF signaling in the ectoderm promotes NSC development. We also describe a novel role for the zinc-finger homeobox gene Zfhx1b (also known as SIP1 or Zeb2) downstream of FGF signaling. Zfhx1b is required for normal neural tube development as well as neural crest specification , is a known downstream transcriptional target of FGF signaling during chick neural induction , and is sufficient to induce a neural cell fate in Xenopus ectoderm [27, 28]. Interestingly, we find that ectopic expression of Zfhx1b in the 7.0-dpc anterior ectoderm is not only sufficient to facilitate the transition from a pNSC to NSC, but can also initiate ectopic neural plate-like tissue formation. Thus, our data suggest that definitive NSC fate specification in the mouse anterior neurectoderm is facilitated by FGF-dependent activation of Zfhx1b.
Transient modulation of FGF and Wnt signaling can regulate gene expression in the mouse ectoderm
As mentioned above, both the Fgf8-/- and the Fgfr1-/- mutant mice display overt neural specification, but subsequent neural plate formation is severely compromised [9, 11]. Thus, we reasoned that FGF signaling may not be required for the initial specification of a neural identity, but it may play a direct role in regulating the transition to a committed neural identity. Using semi-quantitative RT-PCR we observed that direct exposure of the ectoderm to FGF8b enhances and/or stabilizes neural gene expression indicated by the higher levels of Sox2, Nestin, Zfhx1b and Zfhx1a compared to the PBS-injected control embryos within 24 hours (Figure 1C). In contrast, FGF8b injection had no effect on the expression of the mesodermal genes Goosecoid (Gsc) and Brachyury (T), or the pluripotency genes Oct4 and Nanog, as these remain relatively unchanged compared to the PBS injection control (Figure 1C). We also observed a relatively small increase in the expression of Hesx1 in response to FGF8b at 24 hours, a gene that is expressed in the anterior neurectoderm and required for anterior neural plate development . The opposite result was observed when the ectoderm was exposed to Wnt3a, which caused a decrease in neuroectoderm gene expression (Sox2, Zfhx1b) as early as 4 hours post-injection. By approximately 24 hours, Wnt3a had a strong repressive effect on most of the genes that we assayed, except for Oct4 (Figure 1C). Microinjection into the pro-amniotic cavity allowed for the direct exposure of ectoderm cells to exogenous recombinant proteins as opposed to bathing the embryos in growth factor-containing media, which is less sensitive for assessing the effects on neurectodermal gene expression during the relatively short developmental time window we are investigating (Additional file 1A). We also tested whether FGF8b altered the anteroposterior patterning of the neural plate by examining the expression of Otx2 (forebrain and midbrain), En2 (midbrain-hindbrain boundary) and Gbx2 (hindbrain). Acute exposure of the 7.0-dpc ectoderm to FGF8b did not result in any obvious changes in expression of these three genes (Additional file 1B), suggesting that the patterning of relatively broad domains within the neural plate is not significantly affected. However, more detailed analyses using markers with finer expression domains would be necessary to determine if there are more subtle alterations in anteroposterior patterning. In summary, these results indicate that FGF and Wnt signaling can acutely modulate neural cell fate specification within the anterior ectoderm ex vivo.
FGF and Wnt signaling cooperatively regulate neural stem cell development within the mouse embryo
Given our results that exogenous FGF8b and Wnt3a signaling could affect neural gene expression in the ectoderm (Figure 1C), we asked whether these changes in gene expression affect the transition from a pNSC to a NSC identity within the embryo by assaying for NSC colony formation. Fgf2 is not required for mouse neural induction, even though it does regulate neural precursor cell proliferation in the neural tube . On the other hand, Fgf8 expression within the anterior neural ridge is sufficient to induce an anterior forebrain identity , suggesting that FGF8 may be the predominant signal mediating early NSC development in vivo. Thus, we first compared the effectiveness of FGF8b to stimulate definitive NSC clonal colony formation. In Figure 2B we show that FGF2 and FGF8b are equally effective at promoting colony formation, whereas the number of colonies observed in the presence of FGF4 was 60% less than with FGF2, and Wnt3a was incapable of facilitating colony formation (Figure 2B). Moreover, microinjection of FGF8b into the pro-amniotic cavity resulted in an approximately three-fold increase in the number of FGF2-responsive NSCs that were subsequently derived from the anterior neural plate (Figure 2C). These data suggest that modulating the levels of FGF8b on an intact ectoderm is sufficient for regulating NSC development during the formation of the anterior neural plate.
FGF signaling acts through Zfhx1b and is sufficient for the transition from a pNSC to a NSC state in the mouse embryo
In order to determine that Zfhx1b functions downstream of FGF signaling, and mediates the transition between a pNSC and a NSC state, we asked whether the transient over-expression of Zfhx1b can rescue the lack of transition of a pNSC to a NSC following the exposure of the ectoderm to the FGF receptor inhibitor SU5402. Transient transfection of the mouse Zfhx1b DNA construct was effective for inducing increased levels of mRNA transcript and Zfhx1b protein in HEK293T cells (Additional file 3A), and transfection of primary low density neural plate cells post-injection resulted in approximately 75 to 80% expressing cells based on GFP reporter expression (Additional file 3B). As expected, very low numbers of FGF-responsive colonies and relatively high numbers of LIF-responsive colonies were derived from 8.5-dpc neural plate cells (from SU5402 pre-injected embryos) transfected with the EGFP (enhanced green fluorescent protein) control vector (Figure 5C). However, transfection of Zfhx1b in primary dissociated neural plate cells (from SU5402 pre-injected embryos) caused a significant increase (approximately seven-fold) in subsequent FGF-dependent colony formation and a concomitant decrease in LIF-dependent colony formation (Figure 5C). GFP expression was observed in cells within colonies, but due to the transient nature of expression the pattern was often mosaic or of varying intensity by the end of the culture period (data not shown). Thus, ectopic Zfhx1b is sufficient to facilitate the transition from a pNSC to a NSC when FGF signaling is blocked.
Over-expression of Zfhx1b was sufficient to induce an ectopic anterior neural plate/ridge-like structure in approximately 61% of the embryos and in the vast majority of cases it was unilateral (Additional file 4E). In one case the result was a greatly expanded anterior neural plate/ridge-like structure unilaterally (Figure 6C, arrow), but in most other instances a small unilateral anterior neural ridge-like tissue formed at the sight of over-expression. The cells in this expanded region demonstrated strong Otx2 gene expression (Figure 6D, arrow), suggesting they maintain their anterior neural identity within the anterior neural plate. Although most of the affected embryos demonstrated unilateral tissue expansion, in one instance we obtained morphological evidence of bilateral expansion of the anterior neural plate (Additional file 4C,D). These findings are consistent with the observation of an induced ectopic neural tissue in Xenopus ectoderm by XSIP1 over-expression [27, 28]. However, more detailed experiments are required to examine the extent of the morphological differentiation of these expanded neural plate/ridge-like structures. Importantly, we show that the early over-expression of Zfhx1b in ectoderm cells results in an approximately ten-fold increase in the number of FGF-responsive NSCs subsequently present in the 8.5-dpc anterior neural plate, despite the presence of the FGF receptor inhibitor (Figure 6E). The simultaneous derivation of LIF-responsive colonies in this experiment suggests that only those cells that were transfected were able to make the transition, while the many un-transfected cells remained as pNSCs (due to the presence of SU5402), suggesting that the effect is cell autonomous. Thus, Zfhx1b is sufficient to facilitate the precocious transition to a NSC identity in the absence of FGF signaling.
Ectopic Zfhx1b expression is not sufficient to enhance definitive NSC proliferation and colony formation
A possible caveat to the interpretation presented thus far is that FGF signaling is a potent mitogen for NSCs. Therefore, an alternative interpretation is that the transition from pNSC to NSC in vivo may be independent of FGF-Zfhx1b signaling, but that the presence or absence of FGF-Zfhx1b signaling in the neurectoderm can modulate the size of the definitive NSC population via proliferation after the cell fate transition has occurred. Distinguishing between direct effects on cell fate versus selective proliferation can be difficult in this experimental context. However, one reason to suspect that the FGF-Zfhx1b signaling effects are not primarily mediated by selective proliferation is because in the co-injection experiments of DKK1 and SU5402 (Figure 4B), the results show an enhancement of FGF-responsive NSCs in the anterior neural plate compared to control injections despite the pre-exposure of the strong anti-mitogenic effects of the FGF receptor inhibitor SU5402.
The results of several previous studies using ES cell models of mammalian neural induction found that FGF signaling, and downstream MAPK activation, was essential for the establishment of a definitive NSC identity in vitro, one that resembles the NSCs isolated from embryonic neural tube [17, 20, 22, 37, 38]. However, the ES cell model system also revealed that the initial specification of a primitive neural identity can occur independently of FGF signaling and was modulated primarily by the inhibition of well established repressors of vertebrate neural fate, such as BMP, Nodal and Wnt [15, 17, 20, 39, 40]. Importantly, others have observed in the ES cell model [37, 41] and also in chick ectoderm  that FGF-dependent neural tissue formation is preceded by a primitive neurectodermal stage. Our present findings extend these previous results by showing that FGF signaling in the intact mouse ectoderm is required for definitive NSC identity to be established from a pNSC identity. We also demonstrate that the inhibition of Wnt signaling also facilitates the transition of a pNSC to a NSC identity. However, we do not yet know whether this pathway is strictly independent of FGF signaling (that is, without any cross-talk of the signal transduction cascades). Nonetheless, our data indicate that the activation of Zfhx1b gene expression is a common downstream effect of both FGF signaling and Wnt inhibition and is important for promoting the development of a definitive NSC identity in anterior ectoderm cells.
One question that remains to be clearly addressed is whether FGF signaling has an earlier role (that is, during or before gastrulation) in promoting the competence of mouse pluripotent epiblast cells to undergo neural commitment . Indeed, others have demonstrated that the activation of Erk1/2 via autocrine FGF4 signaling in mouse ES cells may be necessary for lineage commitment (either neural or mesodermal) from a pluripotent state , and this is consistent with evidence for an early role of FGF in promoting the neural competence of ectoderm cells in Xenopus and chick (for example, [13, 26]). Technical limitations (in particular embryo viability) prevented us from performing similar microinjection and electroporation experiments in the 6.0-dpc embryos to test this directly. However, we did observe that inhibition of FGF signaling at 7.0 dpc had no obvious effect on the LIF-dependent pNSC population at 7.5 dpc, suggesting that the main role for FGF was in the later transition to a definitive NSC identity. These data are congruent with the recent findings that FGF-induced Erk1/2 signaling controls the transition from an FGF5+ ectoderm identity (perhaps signifying a pNSC identity) to a definitive neural identity in mouse ES cells, and in its absence causes the maintenance or expansion of the ectoderm (presumably neural competent) state . It is likely that the timing of FGF signaling [37, 42] is critical at different stages of development and will be crucial in further elucidating its role in NSC fate specification in mouse.
Our study also reveals a novel role for Zfhx1b in mediating the transition to a definitive NSC identity. Indeed, ectopic expression of Zfhx1b in the absence of FGF signaling was sufficient to promote this lineage transition and as well cause ectopic neural plate tissue. This latter finding is consistent with evidence showing that vertebrate orthologs of Zfhx1b/SIP1 have a critical role in regulating neural induction in Xenopus[27, 28], zebrafish [43, 44], and chick . In the case of chick neural induction, Zfhx1b/SIP1 function is downstream of FGF signaling and is mediated by the transcriptional activator Churchill. Zebrafish Churchill has also been shown to regulate sip1, suggesting that this relationship may be broadly conserved in vertebrates and worth investigating further in mouse, in particular with respect to NSC development.
Zfhx1b/SIP1 is known to interact with Smad proteins and mediate transcriptional repression of the TGFβ/BMP pathway [46–48]. This suggests that perhaps FGF-MAPK activation of Zfhx1b during NSC development acts to inhibit BMP-activated Smad signaling or represses BMP4 gene transcription, similar to what has been reported for FGF-MAPK signaling during Xenopus neural induction [49, 50]. However, the Smad-binding domain is not required for the neural inductive capacity of XSIP1. Interestingly, mammalian Zfhx1b can function as a transcriptional activator and its repressor activity is regulated by post-translational modification . Furthermore, a regulatory neural induction module of the Xenopus Zic1 gene contains Zfhx1b binding sites , as does the mouse Sox1 upstream regulatory domain (L Dang, V Tropepe, unpublished observations), suggesting that this protein may also function by directly activating transcription of genes that may be required for neural induction, such as Zic1. Consistent with this notion, recent evidence demonstrates that in the absence of BMP signaling, FGF provides an instructive signal that is necessary for activating early neural fate regulators, Zic1 and Zic3, in the Xenopus ectoderm .
Fgfr1 and Fgfr2 are expressed in the mouse ectoderm  and the aforementioned targeted deletion experiments suggest that either of these receptors are likely candidates for mediating the FGF signal that promotes NSC development in vivo. However, it remains uncertain which FGF ligand might be the predominant ligand regulating this process. The expression patterns and targeted deletion phenotypes of Fgf4 and Fgf8 appear to indicate that these ligands have an important role in FGF signaling in the ectoderm. Furthermore, Fgf5 is expressed in the early ectoderm , whereas Fgf2 and Fgf1 expression occurs primarily after neural plate formation . Our NSC colony forming experiments revealed that FGF8b was as effective as FGF2 at facilitating colony formation, while FGF4 was significantly less effective. Therefore, Fgf8, and possibly Fgf5, may be the predominant ligand regulating the pNSC to NSC transition in vivo. However, given the substantial cross-activation between various FGF ligands and receptors, further experiments are required to discern the precise role for specific FGF-FGF receptor interactions in vivo.
The orphan nuclear receptor germ cell nuclear factor (GCNF) also regulates the transition from a pNSC to a NSC by repressing Oct4 expression in the neurectoderm . The onset of expression of GCNF seems to parallel that of Zfhx1b, and in the 8.5-dpc neural plate of GCNF-/- embryos, there is an increased number of LIF-responsive pNSCs that persist, along with a concomitant decrease in the number of definitive NSCs . Thus, it is possible that GCNF expression may be modulated downstream of FGF signaling or in a parallel pathway. It will be interesting to determine the regulatory relationship between the GCNF, Zfhx1b and FGF signaling in order to provide further insight into the molecular mechanism of NSC development.
The mouse Zfhx1b/SIP1 knockout demonstrates severe neurodevelopmental malformations . It was reported that the expression of the early neural gene Sox2 was reduced in the neural plate, likely due to the persistence of the expression of E-cadherin, which further compromised the development of the neural plate . Given our findings that Zfhx1b is important for definitive NSC formation in the anterior neurectoderm, we speculate that there is a significant defect in NSC development within the neural plate of the Zfhx1b knockout mouse. Mutations or deletions in the human ZFHX1B gene are strongly associated with Mowat-Wilson syndrome (OMIM 235730), a severe form of mental retardation , suggesting that part of the phenotypic spectrum of this human syndrome may involve a defect in early NSC development.
Our study implicates FGF signaling, and the inhibition of Wnt signaling, in the activation of Zfhx1b gene expression in the mouse anterior ectoderm and in promoting the transition of a pNSC to a definitive NSC cell identity during neural plate formation. The mouse Zfhx1b gene is critical for early neurodevelopment and mutations in the human ortholog have been strongly implicated in causing Mowat-Wilson syndrome, a form a mental retardation. Therefore, our study provides a new perspective on studying the etiology of neurodevelopmental disorders by implicating possible defects in NSC development as a contributing factor underlying disease pathogenesis.
Mouse whole embryo microinjection, electroporation and culture
CD1 mice (Charles River Laboratories, St Constant, Quebec, Canada) were treated in accordance with the regulations on animal experimentation established by the Canadian Council on Animal Care. The experimental procedures were approved by the University of Toronto Animal Care Committee. Embryos from timed-pregnant mice were obtained by dissection from their decidua in 1:1 DMEM:PBS. Reichert's membrane was completely removed using tungsten needle and forceps. Embryos were microinjected using 1.0 mm glass capillary needles (World Precision Instruments, Sarasota, Florida, USA) with the IM 300 Microinjector and subsequently cultured ex vivo in 1:1 DMEM:FBS (Gibco/Stem Cell Technologies, Vancouver, British Columbia, Canada) at 37°C, 5% CO2 for either 4 hours or 24 hours. Recombinant proteins FGF8b (20 ng/ml), FGF2 (20 ng/ml), Wnt3a (100 ng/ml), DKK1 (100 ng/ml), and FGF4 (25 ng/ml) (R&D Systems, Minneapolis, Minnesota, USA) were used according to the manufacturers' instructions. MEK inhibitor, U0126 (Calbiochem-EMD, Gibbstown, New Jersey, USA; gift from Winklbauer Lab) was used at 20 μM in 0.1% DMSO, and FGF receptor inhibitor, SU5402 (Calbiochem; gift from Bonfanti Lab) was used at 5 μM in 0.05% DMSO. 1× PBS as well as 0.05% or 0.1% DMSO was used as injection controls. For the NSC colony-forming assay, standard protocols were used [17, 24] and cells were plated at five cells per microliter in 96-well plates and colonies were counted 14 days after plating. For mouse embryo electroporation [35, 36], midstreak embryos were dissected and injected as described. Plasmids (Zfhx1b/SIP1, NM_015753, OriGene, Rockville, Maryland, USA) or EGFP (from Tam Lab) were injected at 6 μg/μl along with SU5402 (5 μM) in 0.05% DMSO into the amniotic cavity and incubated at room temperature in PBS for at least 5 minutes prior to electroporation. Embryos were positioned between a positive plate electrode (flattened platinum wire) and a negative point electrode (sharpened tungsten wire) with the anterior of the embryo oriented towards the positive plate electrode and were held in place by a holding pipette (hand pulled, cut and microforged), suspended in Tyrode Ringer's Saline pH 7.5. Electroporation was carried out using the BTX Electro Square Porator ECM830 (Harvard Apparatus, Holliston, Massachusetts, USA) at 15V, Path length 0050.6 ms, low voltage mode, 20 ms/pulse, 5 pulses total. Embryos were cultured as described for up to 24 hours. Schematics of mouse embryo sections were adapted from emap: the Edinburgh Mouse Atlas Project .
Transfection was performed using Lipofectamine 2000 (Invitrogen, Carlsbad, California, USA) using 1 μg/well DNA concentrations in 96-well plates. Fluorescence of cells was visualized 24 hours following transfection and colonies derived from tranfected cultures were counted 14 days after plating. The full-length Zfhx1b/SIP1 construct was obtained through Origene (NM_015753) and the EGFP over-expression construct was a gift from Tam Lab.
Neural stem cell colony forming (neurosphere) assay
Anterior neurectoderm (7.5 dpc), anterior neural plate (8.5 dpc) and lateral ganglionic eminence (14.5 dpc) dissections and NSC cultures (cells plated at 5 to 10 cells/μl in the presence of FGF2 and supplemented with heparin were performed as previously described [23, 24]. Means and standard deviations were calculated for the control and experimental treatment groups and statistical differences between groups were determined using a Student's t-test.
Embryos were injected and cultured for 24 hours as described and tissue samples were collected in ice cold lysis buffer containing 10 mM Tris-Cl pH 7.5, 50 mM NaCl, 1 mM EDTA, 1 mM phenylmethanesulfonylfluoride, and Halt phosphatase inhibitor (Thermo Fisher Scientific, Waltham, Massachusetts, USA). The supernatant was subsequently used at 1:1 dilution in protein sample buffer (100 mM Tris-Cl pH 6.8, 30 mM β-mercaptoethanol, 4% SDS, 20% glycerol, 0.01% bromophenol blue). Samples were run on a 10% SDS-PAGE gel and blotted (methanol based transfer) for 2 hours, 30 V constant. Western blotting was performed for Zfhx1b/SIP1 (rabbit anti-SIP1 antibody, 1:1,000; Abcam, Cambridge, Massachusetts, USA), pERK1/2 (rabbit anti-pERK1/2, 1:1,000; Cell Signaling, Danvers, Massachusetts, USA), ERK1/2 (rabbit anti-ERK1/2, 1:1,000; Cell Signaling), and tubulin (mouse anti-Tubulin, 1:1,000; Cell Signaling) following the suggested protocol. Secondary goat anti-rabbit or rabbit anti-goat antibodies conjugated with horse radish peroxidase (Jackson Immunoresearch, West Grove, Pennsylvania, USA) were used at 1:1,000. ECL analysis was used to visualize bands using the ECL Detection Kit (GE Health Care, Piscataway, New Jersey, USA) following the standard protocol.
In situ hybridization
Plasmids containing Zfhx1b/SIP1 (gift from Tylzanowski Lab) and Otx2 (gift from Rossant Lab) were used to generate antisense RNA probes using digoxigenin-11-uridine 5'-triphosphate (Roche, Laval, Quebec, Canada) following the standard protocol. Embryos were fixed in 4% paraformaldehyde at stages indicated, and whole mount in situ hybridization was performed. Digoxigenin-labeled probes were detected with anti-DIG alkaline phosphatase-coupled Fab fragment (1:2,000; Roche). Alkaline phosphatase reaction was performed using BM Purple (Roche).
Quantitative and semi-quantitative PCR
For semi-quantitative PCR, total RNA was extracted with the mini to midi RNA extraction kit (Invitrogen). First strand cDNAs were reverse transcribed from oligo(dT)12-18-primed total RNA (DNase treated, 0.5 μg) using Super-Script III (Invitrogen). Each 25 μl reaction consisted of 1.6× PCR buffer, 1.5 mM MgCl2, 0.2 mM deoxyribonucleotide triphosphates (dNTPs), 0.4 μM each of forward and reverse primers, 0.5 U Platinum Taq DNA polymerase, and diluted cDNA template (1:50). PCR conditions were as follows: 95°C for 3 minutes, 27 to 35 cycles of 95°C for 30 s, 54 to 59°C for 30 s, 72°C for 45 to 60 s. Annealing temperatures and cycle number for each primer pair (Additional file 6) were determined using gradient PCR (MJ Research, Waltham, Massachusetts, USA). Products were resolved on a 1% agarose gel. For real-time RT-PCR the Rotor-Gene 3000 Thermal Cycler (Corbett Research, Cambridge, United Kingdom) was used. First-strand cDNAs were reversed transcribed as described. Each 25 μl reaction consisted of 1.6× PCR buffer, 3 mM MgCl2, 0.2 mM dNTPs, 0.2 μM each of forward and reverse primers (Additional file 6), 0.5 U Platinum Taq DNA polymerase, 1.0× SYBR Green I (Invitrogen), and diluted cDNA template (1:50). Test samples were carried out in duplicate, including a control reaction with template omitted: 95°C for 10 minutes, 50 cycles of 95°C for 15 s, 55°C for 30 s, and 72°C for 45 s. Post-PCR amplification for melt curve analyses was performed by ramping from 72 to 99°C at 0.2°C/s to check the specificity of the amplicons. PCR products were verified on a 1% agarose gel to ensure correct amplification sizes were obtained. Quantification of relative gene expression (using β-actin gene expression as a control) was calculated with Rotor-Gene Software, version 6.0 (Corbett Research).
bone morphogenetic protein
Dulbecco's modified Eagle's medium
enhanced green fluorescent protein
fetal bovine serum
fibroblast growth factor
germ cell nuclear factor
green fluorescent protein
leukemia inhibitory factor
mitogen-activated protein kinase
neural stem cell
primitive neural stem cell
transforming growth factor.
We are very grateful to Dr Patrick Tam and his lab for training, reagents and advice on mouse embryo manipulations and to Dr Bruno Rezende de Souza and Loksum Wong for technical assistance. We also thank Drs Przemko Tylzanowski, Rudi Winklbauer, Janet Rossant and Luca Bonfanti for sharing their reagents, and Drs Rudi Winklbauer and Peter Zandstra for helpful advice throughout the course of this study. This study was supported by grants from NSERC, CFI, OIT and the Connaught Foundation (VT), and by an Ontario Graduate Scholarship and research travel grant from Development (LD).
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