Figure 7

DmGluRA-GAL4 expresses selectively in the mushroom body core neurons. Double stainings for DC0 (magenta) and GFP (green) in brain sections of DmGluRA-GAL4, UAS-mCD8::GFP young adult flies. (A) Within 1 hour after eclosion, anti-GFP brightly stains the α/βc KCs, revealing their cell bodies and dendrites in the calyx (ca) (Aa) and their axons in the pedunculus (ped) (insert in (Ab)) and lobes (Ab). GFP is apparently also present in outer areas of the lobes. (B) Twenty-four hours after eclosion, the intensity of anti-GFP staining appears still high in the α/βc KCs. (C) Ten days after eclosion, GFP expression is less intense but still present in the α/βc neurons of the Drosophila MB. Not all axons from α/βc KCs but only two out of four clonal units are stained (arrows show the absence of the GFP in two groups of cells). So only part of the staining is maintained in the calyx (Ca) whereas intensity appears to still be high in the pedunculus and lobes (Cb). sp, spur. Scale bars: 20 μm.