Figure 6
Morphological features of dendritic arbors of ldaA/ldaA-like and v'ada neurons. (A-D) A pair of ldaA and ldaA-like neurons labeled in different colors by using GFP antibody to visualize the flipout-induced GFP expression (A) (green in (C)) in otherwise CD2-expressing neurons (B) (magenta or white in (C)) in the adult. A merged image (C) and tracings (D) are also shown. Insets indicate high-power images of cell bodies. We did not observe this hemisegment of this animal at a larval stage, so we could not conclude which neuron was derived from larval ldaA. Genotype: yw hsflp/+;UAS-FRT-CD2,y+-FRT-mCD8::GFP/+; C 161/TM2. (E-G) Comparison of arbors of ldaA and ldaA-like neurons. Tracings of representative adult clones of ldaA (E) and ldaA-like (F) in A2 to A5 are shown. These neurons in (E) were designated as ldaA in the adult or simply as 'adult ldaA' because the same hemisegments of individual animals had produced ldaA clones at the larval stage. See the definition of 'ldaA-like' in the text. (G) Plots of total length of dendritic branches of individual larval ldaA (red squares), adult ldaA (blue triangles), and ldaA-like (green diamonds). Horizontal bars indicate means. (H-K) MARCM clones of v'ada in A2 to A5 were found, and those adult flies were aged at 29°C. Neurons at 0 to 6 hours (H), 3 to 4 days (I), and 20 to 21 days (J) after eclosion are shown. The clones were generated as described in the legend of Figure 4. (K) Total length of dendritic branches of individual v'ada arbors of A4 and A5 at 3 to 4 days (red squares) and at 20 to 21 days (blue triangles). Horizontal bars indicate means. Scale bars: 50 μm.