Figure 5

Local interneuron (LNs) do not arise from the anterodorsal cluster. (A) Merge of a few z-sections of the anterodorsal neuroblast clone generated at 0–4 h after larval hatching to show that LNs do not arise from this cluster. L, lateral; D, dorsal. The yellow asterisks in (A4) show that many glomeruli do not have projections from the anterodorsal cells; these cells are therefore unlikely to be LNs. (B) Higher magnification of a single section of a few cells from (A). All cells of this cluster, either GAL4-GH146-labelled or not, do not stain positive with anti-GABA (yellow asterisk and green asterisk, respectively). (C) Ventral cluster of second order olfactory neurons (red dotted line) containing multiglomerular projection neurons; 6–8 of them are marked by GAL4-GH146 (C2) as well as many GABA-ergic cells (C3). (C4) Merge. Scale bars, 10 μm. Genotype: Tub-LexA::GAD/+ or Y; FRTG13, hsFLP, Tub-GAL80/FRTG13, UAS-mCD8, GAL4-GH146; LexAop-rCD2::GFP/+.