Figure 2From: Polarity and intracellular compartmentalization of Drosophila neuronsMushroom body neurons are divided into molecularly distinct compartments. Mushroom body neurons expressing tagged markers were generated by crossing flies containing mushroom body Gal4 drivers with flies carrying UAS-controlled markers. The 201Y Gal4 was used in all panels except L10-YFP and homer-GFP, for which the OK107 Gal4 was used. 201Y drives expression in a large subset of mushroom body neurons [54, 55], and OK107 drives expression in all mushroom body neurons [11] as well as the optic lobe. Brains were dissected from third instar larvae, fixed and analyzed by confocal microscopy. All panels represent confocal projections through the entire mushroom body, except homer-GFP, which shows the cell bodies, dendrites and proximal axons, with distal axons in the inset. Low expression levels of n-syb-YFP expression were generated by using a 2XUAS vector, and low levels of homer-GFP were generated by raising larvae at 18°C. Arrow points to region of proximal axon in which Apc-2 GFP was localized. Scale bar at lower right is 20 μm.Back to article page