Michael Hendricks, Temasek Life Sciences Laboratory
28 May 2007
A couple of clarifications:
1. It is important the embryos in agarose be covered with normal E3 medium for recovery after electroporation, not Ringers solution.
2. Small bubbles should appear on the electrodes during pulsing. Large bubbles (big enough to deform the embryos) or no bubbles indicate non-optimal voltage, unclean electrodes, or something wrong in the circuitry. Voltage may need to be optimized due to possible variation in equipment, connectors, etc.
Neural Development
important points in using this technqie
28 May 2007
A couple of clarifications:
1. It is important the embryos in agarose be covered with normal E3 medium for recovery after electroporation, not Ringers solution.
2. Small bubbles should appear on the electrodes during pulsing. Large bubbles (big enough to deform the embryos) or no bubbles indicate non-optimal voltage, unclean electrodes, or something wrong in the circuitry. Voltage may need to be optimized due to possible variation in equipment, connectors, etc.
Competing interests
None declared