Figure 5

Unilateral VCN removal at P10 does not induce projections to the ipsilateral MNTB. Labeled calyces are seen in the contralateral MNTB (MNTBc) of wild type (A and C) and EphB2^−/− mice (E and G) after removing the VCN at P10, but not in the ipsilateral MNTB (MNTBi) of either mouse (B, D, F, H). The mean ipsilateral/contralateral (I/C) ratios did not differ after unilateral VCN removal at P10 (I, P > 0.05). Taken together, deafferentation-induced innervation of the ipsilateral MNTB decreases postnatally in wild type and EphB2^−/− mice (J). EphB2^−/− mice have more ipsilateral sprouting after unilateral deafferentation at P2 and P7, but not at P10, when the critical period for this process has ended. Y-axis values in panel (J) are I/C ratios represented as a percentage of the I/C ratio obtained after cochlea removal in wild type mice at P2. P2 data is from Nakamura et al., 2012. Scale bar in panel (A) represents 200 μm and also applies to panels (B-H).