Figure 5

Preoptic glia and most tuberal and mammillary neurons are of Shh descent. (A) Schematic distribution of tyrosine hydroxylase (Th)-expressing cells (green dots) on a sagittal view of the hypothalamus. (B-P) Transverse sections of adult mouse brains TM8.0 (B, E, H, K, N), TM9.5 (C, F, I, L, O) and TM11.5 (D, G, J, M, P) labeled for Shh lineage (β-gal, red) and Th (green). (B-D) The preoptic region contains large numbers of late-stage fate-mapped cells with glia-like morphology. (E-G) The anterior hypothalamus contains very few fate-mapped cells at all stages. (H-M) Many fate-mapped cells are located in the tuberal area at all stages, especially in the ventromedial nucleus (VMH). (N-P) Early-stage fate-mapped cells (TM8.5) also contribute strongly to the mammillary body. A13, dopaminergic cell group A13; ac, anterior commissure; AHA, anterior hypothalamic region; ARH, arcuate nucleus; DMH, dorsomedial nucleus; opt, optic tract; MBO, mammillary body; ME, median eminence; Ox, optic chiasm; PH, posterior hypothalamus; PVH, paraventricular hypothalamic nucleus; SCH, suprachiasmatic nucleus; VMH, ventromedial nucleus. Scale bar: 800 μm.