Figure 5

Mitochondria and Apc2-GFP localize to dendrite branch points in larvae with mixed dendritic microtubules. (A) Control (rtnl2) or Kap3 RNA hairpins were expressed in neurons together with mCD8-RFP, dicer2, and mitoGFP. Images of neurons in living larvae were acquired and mitochondria distribution in dendrites was analyzed. Arrows point to the cell body of ddaE and arrowheads to mitochondria at branch points along the comb dendrite. (B) Control (rtnl2) or Kap3 RNA hairpins were expressed with Apc2-GFP, dicer2, and mCD8-GFP in class I da neurons with 221-Gal4. Example images are shown; arrows point to ddaE cell bodies and arrowheads to punctae of Apc2-GFP at branch points along the main trunk of the ddaE comb dendrite. (C) In the left panel, the number of mitochondria per 100 μm of dendrite length is shown. The length of the main trunk of the ddaE neuron was measured, and the number of mitochondria in this part of the dendrite arbor was counted. A value was generated for each cell analyzed, and the average of these is shown in the graph. Error bars are standard deviations of these averages. In the middle panel the percentage of branch points along the comb dendrite of ddaE occupied by mitochondria is shown. Again, percentages were generated for each neuron and the averages and standard deviations were graphed. The right panel shows a similar analysis of branch points occupied by Apc2-GFP. In all graphs the number of animals analyzed for each genotype is shown on the bar.